IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay

Masato Kadoya, Kenichi Kaida, Haruki Koike, Hiroshi Takazaki, Hidenori Ogata, Kota Moriguchi, Jun Shimizu, Eiichiro Nagata, Shunya Takizawa, Atsuro Chiba, Ryo Yamasaki, Jun ichi Kira, Gen Sobue, Katsunori Ikewaki

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We aimed to validate the diagnostic utility of enzyme-linked immunosorbent assay (ELISA) for the detection of anti-neurofascin (NF) 155 antibody in 191 patients with chronic inflammatory demyelinating polyneuropathy (CIDP). Human NF155-based ELISA clearly distinguished between anti-NF155 antibody-positive and -negative sera. Fifteen CIDP patients (8%) were IgG4 anti-human NF155 antibody-positive, which were confirmed by western blot, cell-based assay and immunohistochemical study. None of disease controls or healthy subjects had positive results. Clinical presentation of IgG4 anti-NF155 antibody-positive patients was consistent with those in previous reports. This ELISA combined with determination of the IgG4 subclass is useful in screening for anti-NF155 antibodies.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalJournal of Neuroimmunology
Volume301
DOIs
Publication statusPublished - Dec 15 2016

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Chronic Inflammatory Demyelinating Polyradiculoneuropathy
Anti-Idiotypic Antibodies
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Antibodies
Healthy Volunteers
Western Blotting
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy : Clinical significance and diagnostic utility of a conventional assay. / Kadoya, Masato; Kaida, Kenichi; Koike, Haruki; Takazaki, Hiroshi; Ogata, Hidenori; Moriguchi, Kota; Shimizu, Jun; Nagata, Eiichiro; Takizawa, Shunya; Chiba, Atsuro; Yamasaki, Ryo; Kira, Jun ichi; Sobue, Gen; Ikewaki, Katsunori.

In: Journal of Neuroimmunology, Vol. 301, 15.12.2016, p. 16-22.

Research output: Contribution to journalArticle

Kadoya, Masato ; Kaida, Kenichi ; Koike, Haruki ; Takazaki, Hiroshi ; Ogata, Hidenori ; Moriguchi, Kota ; Shimizu, Jun ; Nagata, Eiichiro ; Takizawa, Shunya ; Chiba, Atsuro ; Yamasaki, Ryo ; Kira, Jun ichi ; Sobue, Gen ; Ikewaki, Katsunori. / IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy : Clinical significance and diagnostic utility of a conventional assay. In: Journal of Neuroimmunology. 2016 ; Vol. 301. pp. 16-22.
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AU - Nagata, Eiichiro

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