IL-10 is involved in the protective effect of dibutyryl cyclic adenosine monophosphate on endotoxin-induced inflammatory liver injury

T. Arai, K. Hiromatsu, N. Kobayashi, M. Takano, H. Ishida, Y. Nimura, Y. Yoshikai

    Research output: Contribution to journalArticle

    94 Citations (Scopus)

    Abstract

    The effects of exogenous cAMP, dibutyryl cAMP (DBcAMP) on LPS-induced liver injury were examined in mice made hypersensitive to LPS by treatment with i.v. injection of Propionibacterium acnes. In vivo administration of DBcAMP significantly protected P. acnes-treated mice from LPS-induced liver injury, including apoptosis of hepatocytes. DBcAMP significantly increased circulating IL-10 level in correlation with suppression of the TNF-α level after LPS challenge in P. acnes-treated mice. Treatment with anti-IL-10 mAb abrogated the protective effect of DBcAMP on LPS-induced liver injury. Similar to in vivo findings, addition of DBcAMP to in vitro culture of liver adherent cells from P. acnes-treated mice enhanced IL-10 synthesis after LPS stimulation. These results suggest that the increment in IL-10 production by liver adherent cells is involved in the protective effect of DBcAMP on LPS- induced inflammatory liver injury.

    Original languageEnglish
    Pages (from-to)5743-5749
    Number of pages7
    JournalJournal of Immunology
    Volume155
    Issue number12
    Publication statusPublished - Dec 20 1995

    Fingerprint

    Endotoxins
    Cyclic AMP
    Interleukin-10
    Propionibacterium acnes
    Liver
    Wounds and Injuries
    Hepatocytes
    Apoptosis
    Injections
    Therapeutics

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Arai, T., Hiromatsu, K., Kobayashi, N., Takano, M., Ishida, H., Nimura, Y., & Yoshikai, Y. (1995). IL-10 is involved in the protective effect of dibutyryl cyclic adenosine monophosphate on endotoxin-induced inflammatory liver injury. Journal of Immunology, 155(12), 5743-5749.

    IL-10 is involved in the protective effect of dibutyryl cyclic adenosine monophosphate on endotoxin-induced inflammatory liver injury. / Arai, T.; Hiromatsu, K.; Kobayashi, N.; Takano, M.; Ishida, H.; Nimura, Y.; Yoshikai, Y.

    In: Journal of Immunology, Vol. 155, No. 12, 20.12.1995, p. 5743-5749.

    Research output: Contribution to journalArticle

    Arai, T, Hiromatsu, K, Kobayashi, N, Takano, M, Ishida, H, Nimura, Y & Yoshikai, Y 1995, 'IL-10 is involved in the protective effect of dibutyryl cyclic adenosine monophosphate on endotoxin-induced inflammatory liver injury', Journal of Immunology, vol. 155, no. 12, pp. 5743-5749.
    Arai T, Hiromatsu K, Kobayashi N, Takano M, Ishida H, Nimura Y et al. IL-10 is involved in the protective effect of dibutyryl cyclic adenosine monophosphate on endotoxin-induced inflammatory liver injury. Journal of Immunology. 1995 Dec 20;155(12):5743-5749.
    Arai, T. ; Hiromatsu, K. ; Kobayashi, N. ; Takano, M. ; Ishida, H. ; Nimura, Y. ; Yoshikai, Y. / IL-10 is involved in the protective effect of dibutyryl cyclic adenosine monophosphate on endotoxin-induced inflammatory liver injury. In: Journal of Immunology. 1995 ; Vol. 155, No. 12. pp. 5743-5749.
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    abstract = "The effects of exogenous cAMP, dibutyryl cAMP (DBcAMP) on LPS-induced liver injury were examined in mice made hypersensitive to LPS by treatment with i.v. injection of Propionibacterium acnes. In vivo administration of DBcAMP significantly protected P. acnes-treated mice from LPS-induced liver injury, including apoptosis of hepatocytes. DBcAMP significantly increased circulating IL-10 level in correlation with suppression of the TNF-α level after LPS challenge in P. acnes-treated mice. Treatment with anti-IL-10 mAb abrogated the protective effect of DBcAMP on LPS-induced liver injury. Similar to in vivo findings, addition of DBcAMP to in vitro culture of liver adherent cells from P. acnes-treated mice enhanced IL-10 synthesis after LPS stimulation. These results suggest that the increment in IL-10 production by liver adherent cells is involved in the protective effect of DBcAMP on LPS- induced inflammatory liver injury.",
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    AU - Arai, T.

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    AU - Kobayashi, N.

    AU - Takano, M.

    AU - Ishida, H.

    AU - Nimura, Y.

    AU - Yoshikai, Y.

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    AB - The effects of exogenous cAMP, dibutyryl cAMP (DBcAMP) on LPS-induced liver injury were examined in mice made hypersensitive to LPS by treatment with i.v. injection of Propionibacterium acnes. In vivo administration of DBcAMP significantly protected P. acnes-treated mice from LPS-induced liver injury, including apoptosis of hepatocytes. DBcAMP significantly increased circulating IL-10 level in correlation with suppression of the TNF-α level after LPS challenge in P. acnes-treated mice. Treatment with anti-IL-10 mAb abrogated the protective effect of DBcAMP on LPS-induced liver injury. Similar to in vivo findings, addition of DBcAMP to in vitro culture of liver adherent cells from P. acnes-treated mice enhanced IL-10 synthesis after LPS stimulation. These results suggest that the increment in IL-10 production by liver adherent cells is involved in the protective effect of DBcAMP on LPS- induced inflammatory liver injury.

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