Il-10 production in circulating T cells differs between allergen-induced isolated early and dual asthmatic responders

Koichiro Matsumoto, Gail M. Gauvreau, Tracy Rerecich, Richard M. Watson, Lorna J. Wood, Paul M. O'Byrne

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: IL-10 is an anti-inflammatory cytokine released from various cells, including T cells. The role of IL-10 in asthma pathogenesis remains uncertain. Allergen inhalation by atopic asthmatic subjects results in 2 bronchoconstrictor phenotypes: isolated early response and dual response. Persistence of allergen-induced airway inflammation is a feature of dual responders. Objectives: The kinetics of IL-10 production in circulating T cells were investigated to examine a potential role of IL-10 in allergen-induced responses and airway inflammation. Methods: Fourteen subjects with mild asthma (7 isolated early and 7 dual responders) were challenged with allergen. PBMCs taken before and 24 hours after allergen challenge were processed for intracellular IL-10 staining with fluorescent-conjugated anti-IL-10 antibody. The frequency of IL-10-producing cells was assessed for CD4+ and CD8+ T-cell subsets by using flow cytometry. Results: Before allergen administration, the frequency of IL10-producing CD4+ cells was significantly higher in dual than in isolated early responders. IL-10-producing CD4+ cells significantly increased after allergen in early responders, whereas IL-10-producing CD4+ cells significantly decreased in dual responders. Simultaneous assessments of IL-5-producing T cells did not show any differences between each group before or after allergen administration. Conclusions: These results suggest that the contrasting profiles of IL-10 production may be associated with the different time course of allergen-induced airway inflammation between allergen-induced early and dual responders.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalJournal of Allergy and Clinical Immunology
Volume109
Issue number2
DOIs
Publication statusPublished - Jan 1 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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