IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection

Hitoshi Nishimura, Kenji Hiromatsu, Noritada Kobayashi, Kenneth H. Grabstein, Raymond Paxton, Kazuo Sugamura, Jeffrey A. Bluestone, Yasunobu Yoshikai

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    We have previously shown evidence for the early recruitment of γδ T cells during the disease course of primary infections with Listeria monocytogenes or Salmonella choleraesuis in mice. Since γδ T cells at this stage of the disease do not produce IL-2, the growth factor for the γδ T cells remains unknown. IL-15 is a novel cytokine that uses β- and γ-chain of IL-2R for signal transduction, and is produced by activated monocytes/macrophages. In this study, we investigated the proliferative activity of IL-15 for γδ T cells appearing after primary infection with S. choleraesuis 31N-1. The γδ T cells, which expressed β- and γ-chains of IL-2R, proliferated in the presence of rIL-15 and produced appreciable levels of γ-IFN and IL-4. Addition of anti-IL-2Rβ mAb significantly inhibited the IL-15-induced proliferation of the γδ T cells. Furthermore, the γδ T cells produced γ-IFN in response to monocyte/macrophage cell line, J774A.1 infected with S. choleraesuis, which expressed an abundant level of IL-15 mRNA. This cytokine production was inhibited significantly by anti-IL-15 Ab. Taken together, these results suggest that IL-15 derived from infected macrophages may contribute to the early activation of γδ T cells during salmonellosis.

    Original languageEnglish
    Pages (from-to)663-669
    Number of pages7
    JournalJournal of Immunology
    Issue number2
    Publication statusPublished - Jan 15 1996

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

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    Nishimura, H., Hiromatsu, K., Kobayashi, N., Grabstein, K. H., Paxton, R., Sugamura, K., Bluestone, J. A., & Yoshikai, Y. (1996). IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection. Journal of Immunology, 156(2), 663-669.