IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection

Hitoshi Nishimura, Kenji Hiromatsu, Noritada Kobayashi, Kenneth H. Grabstein, Raymond Paxton, Kazuo Sugamura, Jeffrey A. Bluestone, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    144 Citations (Scopus)

    Abstract

    We have previously shown evidence for the early recruitment of γδ T cells during the disease course of primary infections with Listeria monocytogenes or Salmonella choleraesuis in mice. Since γδ T cells at this stage of the disease do not produce IL-2, the growth factor for the γδ T cells remains unknown. IL-15 is a novel cytokine that uses β- and γ-chain of IL-2R for signal transduction, and is produced by activated monocytes/macrophages. In this study, we investigated the proliferative activity of IL-15 for γδ T cells appearing after primary infection with S. choleraesuis 31N-1. The γδ T cells, which expressed β- and γ-chains of IL-2R, proliferated in the presence of rIL-15 and produced appreciable levels of γ-IFN and IL-4. Addition of anti-IL-2Rβ mAb significantly inhibited the IL-15-induced proliferation of the γδ T cells. Furthermore, the γδ T cells produced γ-IFN in response to monocyte/macrophage cell line, J774A.1 infected with S. choleraesuis, which expressed an abundant level of IL-15 mRNA. This cytokine production was inhibited significantly by anti-IL-15 Ab. Taken together, these results suggest that IL-15 derived from infected macrophages may contribute to the early activation of γδ T cells during salmonellosis.

    Original languageEnglish
    Pages (from-to)663-669
    Number of pages7
    JournalJournal of Immunology
    Volume156
    Issue number2
    Publication statusPublished - Jan 15 1996

    Fingerprint

    Interleukin-15
    Salmonella Infections
    Interleukin-2
    T-Lymphocytes
    Macrophages
    Monocytes
    Cytokines
    Listeria monocytogenes
    Infection
    Salmonella
    Interleukin-4
    Signal Transduction
    Cell Line
    Messenger RNA

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Nishimura, H., Hiromatsu, K., Kobayashi, N., Grabstein, K. H., Paxton, R., Sugamura, K., ... Yoshikai, Y. (1996). IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection. Journal of Immunology, 156(2), 663-669.

    IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection. / Nishimura, Hitoshi; Hiromatsu, Kenji; Kobayashi, Noritada; Grabstein, Kenneth H.; Paxton, Raymond; Sugamura, Kazuo; Bluestone, Jeffrey A.; Yoshikai, Yasunobu.

    In: Journal of Immunology, Vol. 156, No. 2, 15.01.1996, p. 663-669.

    Research output: Contribution to journalArticle

    Nishimura, H, Hiromatsu, K, Kobayashi, N, Grabstein, KH, Paxton, R, Sugamura, K, Bluestone, JA & Yoshikai, Y 1996, 'IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection', Journal of Immunology, vol. 156, no. 2, pp. 663-669.
    Nishimura H, Hiromatsu K, Kobayashi N, Grabstein KH, Paxton R, Sugamura K et al. IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection. Journal of Immunology. 1996 Jan 15;156(2):663-669.
    Nishimura, Hitoshi ; Hiromatsu, Kenji ; Kobayashi, Noritada ; Grabstein, Kenneth H. ; Paxton, Raymond ; Sugamura, Kazuo ; Bluestone, Jeffrey A. ; Yoshikai, Yasunobu. / IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection. In: Journal of Immunology. 1996 ; Vol. 156, No. 2. pp. 663-669.
    @article{115ae261d58043ec87b8c2f87d9b80eb,
    title = "IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection",
    abstract = "We have previously shown evidence for the early recruitment of γδ T cells during the disease course of primary infections with Listeria monocytogenes or Salmonella choleraesuis in mice. Since γδ T cells at this stage of the disease do not produce IL-2, the growth factor for the γδ T cells remains unknown. IL-15 is a novel cytokine that uses β- and γ-chain of IL-2R for signal transduction, and is produced by activated monocytes/macrophages. In this study, we investigated the proliferative activity of IL-15 for γδ T cells appearing after primary infection with S. choleraesuis 31N-1. The γδ T cells, which expressed β- and γ-chains of IL-2R, proliferated in the presence of rIL-15 and produced appreciable levels of γ-IFN and IL-4. Addition of anti-IL-2Rβ mAb significantly inhibited the IL-15-induced proliferation of the γδ T cells. Furthermore, the γδ T cells produced γ-IFN in response to monocyte/macrophage cell line, J774A.1 infected with S. choleraesuis, which expressed an abundant level of IL-15 mRNA. This cytokine production was inhibited significantly by anti-IL-15 Ab. Taken together, these results suggest that IL-15 derived from infected macrophages may contribute to the early activation of γδ T cells during salmonellosis.",
    author = "Hitoshi Nishimura and Kenji Hiromatsu and Noritada Kobayashi and Grabstein, {Kenneth H.} and Raymond Paxton and Kazuo Sugamura and Bluestone, {Jeffrey A.} and Yasunobu Yoshikai",
    year = "1996",
    month = "1",
    day = "15",
    language = "English",
    volume = "156",
    pages = "663--669",
    journal = "Journal of Immunology",
    issn = "0022-1767",
    publisher = "American Association of Immunologists",
    number = "2",

    }

    TY - JOUR

    T1 - IL-15 is a novel growth factor for murine γδ T cells induced by Salmonella infection

    AU - Nishimura, Hitoshi

    AU - Hiromatsu, Kenji

    AU - Kobayashi, Noritada

    AU - Grabstein, Kenneth H.

    AU - Paxton, Raymond

    AU - Sugamura, Kazuo

    AU - Bluestone, Jeffrey A.

    AU - Yoshikai, Yasunobu

    PY - 1996/1/15

    Y1 - 1996/1/15

    N2 - We have previously shown evidence for the early recruitment of γδ T cells during the disease course of primary infections with Listeria monocytogenes or Salmonella choleraesuis in mice. Since γδ T cells at this stage of the disease do not produce IL-2, the growth factor for the γδ T cells remains unknown. IL-15 is a novel cytokine that uses β- and γ-chain of IL-2R for signal transduction, and is produced by activated monocytes/macrophages. In this study, we investigated the proliferative activity of IL-15 for γδ T cells appearing after primary infection with S. choleraesuis 31N-1. The γδ T cells, which expressed β- and γ-chains of IL-2R, proliferated in the presence of rIL-15 and produced appreciable levels of γ-IFN and IL-4. Addition of anti-IL-2Rβ mAb significantly inhibited the IL-15-induced proliferation of the γδ T cells. Furthermore, the γδ T cells produced γ-IFN in response to monocyte/macrophage cell line, J774A.1 infected with S. choleraesuis, which expressed an abundant level of IL-15 mRNA. This cytokine production was inhibited significantly by anti-IL-15 Ab. Taken together, these results suggest that IL-15 derived from infected macrophages may contribute to the early activation of γδ T cells during salmonellosis.

    AB - We have previously shown evidence for the early recruitment of γδ T cells during the disease course of primary infections with Listeria monocytogenes or Salmonella choleraesuis in mice. Since γδ T cells at this stage of the disease do not produce IL-2, the growth factor for the γδ T cells remains unknown. IL-15 is a novel cytokine that uses β- and γ-chain of IL-2R for signal transduction, and is produced by activated monocytes/macrophages. In this study, we investigated the proliferative activity of IL-15 for γδ T cells appearing after primary infection with S. choleraesuis 31N-1. The γδ T cells, which expressed β- and γ-chains of IL-2R, proliferated in the presence of rIL-15 and produced appreciable levels of γ-IFN and IL-4. Addition of anti-IL-2Rβ mAb significantly inhibited the IL-15-induced proliferation of the γδ T cells. Furthermore, the γδ T cells produced γ-IFN in response to monocyte/macrophage cell line, J774A.1 infected with S. choleraesuis, which expressed an abundant level of IL-15 mRNA. This cytokine production was inhibited significantly by anti-IL-15 Ab. Taken together, these results suggest that IL-15 derived from infected macrophages may contribute to the early activation of γδ T cells during salmonellosis.

    UR - http://www.scopus.com/inward/record.url?scp=0030026047&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0030026047&partnerID=8YFLogxK

    M3 - Article

    C2 - 8543818

    AN - SCOPUS:0030026047

    VL - 156

    SP - 663

    EP - 669

    JO - Journal of Immunology

    JF - Journal of Immunology

    SN - 0022-1767

    IS - 2

    ER -