IL-3 augments TCR-mediated responses of type 2 CD4 T cells

Yoshiyuki Dan, Yoshinori Katakura, Akio Ametani, Shuichi Kaminogawa, Yoshihiro Asano

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A subset of type 2, but not type 1, CD4 T cell clones expresses IL-3R and can be stimulated by IL-3. Expression of IL-3R on these type 2 T cell clones is induced by TCR stimulation, and subsequent stimulation by IL-3 augmented the proliferation of and IL-4 production by these cells. This augmented response is inhibited by anti-IL-4 mAb, suggesting the involvement of IL-4 in this response. In place of TCR stimulation, treatment of these type 2 CD4 T cell clones with PMA rendered them responsive to further stimulation of proliferation by IL-3, indicating the cooperation between the IL-3R-elicited signals and PKC-mediated signals in stimulating proliferation. Although the augmentation of the TCR-mediated proliferative response by IL-3 was mainly due to the increased production of IL-4, we also demonstrated the presence of IL-4-independent mechanism mediating the response to IL-3. In situ, we found that splenic T cells could be induced to respond to IL-3 by TCR stimulation. Thus, IL-3 can stimulate a specific population of T cells and influence the immune response.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalJournal of Immunology
Volume156
Issue number1
Publication statusPublished - Jan 1 1996
Externally publishedYes

Fingerprint

Interleukin-3
Interleukin-4
T-Lymphocytes
Clone Cells
Population

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Dan, Y., Katakura, Y., Ametani, A., Kaminogawa, S., & Asano, Y. (1996). IL-3 augments TCR-mediated responses of type 2 CD4 T cells. Journal of Immunology, 156(1), 27-34.

IL-3 augments TCR-mediated responses of type 2 CD4 T cells. / Dan, Yoshiyuki; Katakura, Yoshinori; Ametani, Akio; Kaminogawa, Shuichi; Asano, Yoshihiro.

In: Journal of Immunology, Vol. 156, No. 1, 01.01.1996, p. 27-34.

Research output: Contribution to journalArticle

Dan, Y, Katakura, Y, Ametani, A, Kaminogawa, S & Asano, Y 1996, 'IL-3 augments TCR-mediated responses of type 2 CD4 T cells', Journal of Immunology, vol. 156, no. 1, pp. 27-34.
Dan Y, Katakura Y, Ametani A, Kaminogawa S, Asano Y. IL-3 augments TCR-mediated responses of type 2 CD4 T cells. Journal of Immunology. 1996 Jan 1;156(1):27-34.
Dan, Yoshiyuki ; Katakura, Yoshinori ; Ametani, Akio ; Kaminogawa, Shuichi ; Asano, Yoshihiro. / IL-3 augments TCR-mediated responses of type 2 CD4 T cells. In: Journal of Immunology. 1996 ; Vol. 156, No. 1. pp. 27-34.
@article{8c141eaa22b84f72a918af28998863a5,
title = "IL-3 augments TCR-mediated responses of type 2 CD4 T cells",
abstract = "A subset of type 2, but not type 1, CD4 T cell clones expresses IL-3R and can be stimulated by IL-3. Expression of IL-3R on these type 2 T cell clones is induced by TCR stimulation, and subsequent stimulation by IL-3 augmented the proliferation of and IL-4 production by these cells. This augmented response is inhibited by anti-IL-4 mAb, suggesting the involvement of IL-4 in this response. In place of TCR stimulation, treatment of these type 2 CD4 T cell clones with PMA rendered them responsive to further stimulation of proliferation by IL-3, indicating the cooperation between the IL-3R-elicited signals and PKC-mediated signals in stimulating proliferation. Although the augmentation of the TCR-mediated proliferative response by IL-3 was mainly due to the increased production of IL-4, we also demonstrated the presence of IL-4-independent mechanism mediating the response to IL-3. In situ, we found that splenic T cells could be induced to respond to IL-3 by TCR stimulation. Thus, IL-3 can stimulate a specific population of T cells and influence the immune response.",
author = "Yoshiyuki Dan and Yoshinori Katakura and Akio Ametani and Shuichi Kaminogawa and Yoshihiro Asano",
year = "1996",
month = "1",
day = "1",
language = "English",
volume = "156",
pages = "27--34",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "1",

}

TY - JOUR

T1 - IL-3 augments TCR-mediated responses of type 2 CD4 T cells

AU - Dan, Yoshiyuki

AU - Katakura, Yoshinori

AU - Ametani, Akio

AU - Kaminogawa, Shuichi

AU - Asano, Yoshihiro

PY - 1996/1/1

Y1 - 1996/1/1

N2 - A subset of type 2, but not type 1, CD4 T cell clones expresses IL-3R and can be stimulated by IL-3. Expression of IL-3R on these type 2 T cell clones is induced by TCR stimulation, and subsequent stimulation by IL-3 augmented the proliferation of and IL-4 production by these cells. This augmented response is inhibited by anti-IL-4 mAb, suggesting the involvement of IL-4 in this response. In place of TCR stimulation, treatment of these type 2 CD4 T cell clones with PMA rendered them responsive to further stimulation of proliferation by IL-3, indicating the cooperation between the IL-3R-elicited signals and PKC-mediated signals in stimulating proliferation. Although the augmentation of the TCR-mediated proliferative response by IL-3 was mainly due to the increased production of IL-4, we also demonstrated the presence of IL-4-independent mechanism mediating the response to IL-3. In situ, we found that splenic T cells could be induced to respond to IL-3 by TCR stimulation. Thus, IL-3 can stimulate a specific population of T cells and influence the immune response.

AB - A subset of type 2, but not type 1, CD4 T cell clones expresses IL-3R and can be stimulated by IL-3. Expression of IL-3R on these type 2 T cell clones is induced by TCR stimulation, and subsequent stimulation by IL-3 augmented the proliferation of and IL-4 production by these cells. This augmented response is inhibited by anti-IL-4 mAb, suggesting the involvement of IL-4 in this response. In place of TCR stimulation, treatment of these type 2 CD4 T cell clones with PMA rendered them responsive to further stimulation of proliferation by IL-3, indicating the cooperation between the IL-3R-elicited signals and PKC-mediated signals in stimulating proliferation. Although the augmentation of the TCR-mediated proliferative response by IL-3 was mainly due to the increased production of IL-4, we also demonstrated the presence of IL-4-independent mechanism mediating the response to IL-3. In situ, we found that splenic T cells could be induced to respond to IL-3 by TCR stimulation. Thus, IL-3 can stimulate a specific population of T cells and influence the immune response.

UR - http://www.scopus.com/inward/record.url?scp=0029671320&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029671320&partnerID=8YFLogxK

M3 - Article

VL - 156

SP - 27

EP - 34

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -