Abstract
We have investigated the effects of interleukin-4 (IL-4) on the proliferation of chronic myelomonocytic leukemia (CMMoL) cells in the chronic and leukemic transformation phases in vitro. CMMoL cells formed colonies spontaneously in both phases. IL-4 suppressed the spontaneous growth in the chronic phase, but on the other hand, stimulated colony formation in the leukemic transformation phase. Anti-IL-6 antibody inhibited spontaneous colony formation in both phases. CMMoL cells in both phases produced high levels of IL-6, compared with those produced by acute myelogenous leukemia (AML) cells showing myelomonocytic differentiation and normal monocytes. IL-4 suppressed the IL-6 production by CMMoL cells in both phases. None of anti-IL-6, anti-macrophage colony-stimulating factor (M-CSF), anti-granulocyte-macrophage colony-stimulating factor (GM-CSF), anti-tumor necrosis factor-α (TNF-α) and anti-IL-1-β antibodies inhibited IL-4-stimulated colony formation. These results suggest that IL-4 directly stimulates the growth of CMMoL cells once leukemic transformation has occurred and that the therapeutic use of IL-4 for CMMoL should he viewed with caution, especially in the leukemic transformation phase.
| Original language | English |
|---|---|
| Pages (from-to) | 1056-1059 |
| Number of pages | 4 |
| Journal | Leukemia |
| Volume | 9 |
| Issue number | 6 |
| Publication status | Published - Jan 1 1995 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Hematology
- Oncology
- Cancer Research
Cite this
IL-4 stimulates the growth of chronic myelomonocytic leukemia cells (CMMoL) once leukemic transformation has occurred. / Yanagisawa, K.; Hatta, N.; Watanabe, I.; Horiuchi, Takahiko; Hasegawa, H.; Fujita, S.
In: Leukemia, Vol. 9, No. 6, 01.01.1995, p. 1056-1059.Research output: Contribution to journal › Article
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TY - JOUR
T1 - IL-4 stimulates the growth of chronic myelomonocytic leukemia cells (CMMoL) once leukemic transformation has occurred
AU - Yanagisawa, K.
AU - Hatta, N.
AU - Watanabe, I.
AU - Horiuchi, Takahiko
AU - Hasegawa, H.
AU - Fujita, S.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - We have investigated the effects of interleukin-4 (IL-4) on the proliferation of chronic myelomonocytic leukemia (CMMoL) cells in the chronic and leukemic transformation phases in vitro. CMMoL cells formed colonies spontaneously in both phases. IL-4 suppressed the spontaneous growth in the chronic phase, but on the other hand, stimulated colony formation in the leukemic transformation phase. Anti-IL-6 antibody inhibited spontaneous colony formation in both phases. CMMoL cells in both phases produced high levels of IL-6, compared with those produced by acute myelogenous leukemia (AML) cells showing myelomonocytic differentiation and normal monocytes. IL-4 suppressed the IL-6 production by CMMoL cells in both phases. None of anti-IL-6, anti-macrophage colony-stimulating factor (M-CSF), anti-granulocyte-macrophage colony-stimulating factor (GM-CSF), anti-tumor necrosis factor-α (TNF-α) and anti-IL-1-β antibodies inhibited IL-4-stimulated colony formation. These results suggest that IL-4 directly stimulates the growth of CMMoL cells once leukemic transformation has occurred and that the therapeutic use of IL-4 for CMMoL should he viewed with caution, especially in the leukemic transformation phase.
AB - We have investigated the effects of interleukin-4 (IL-4) on the proliferation of chronic myelomonocytic leukemia (CMMoL) cells in the chronic and leukemic transformation phases in vitro. CMMoL cells formed colonies spontaneously in both phases. IL-4 suppressed the spontaneous growth in the chronic phase, but on the other hand, stimulated colony formation in the leukemic transformation phase. Anti-IL-6 antibody inhibited spontaneous colony formation in both phases. CMMoL cells in both phases produced high levels of IL-6, compared with those produced by acute myelogenous leukemia (AML) cells showing myelomonocytic differentiation and normal monocytes. IL-4 suppressed the IL-6 production by CMMoL cells in both phases. None of anti-IL-6, anti-macrophage colony-stimulating factor (M-CSF), anti-granulocyte-macrophage colony-stimulating factor (GM-CSF), anti-tumor necrosis factor-α (TNF-α) and anti-IL-1-β antibodies inhibited IL-4-stimulated colony formation. These results suggest that IL-4 directly stimulates the growth of CMMoL cells once leukemic transformation has occurred and that the therapeutic use of IL-4 for CMMoL should he viewed with caution, especially in the leukemic transformation phase.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029036446&partnerID=8YFLogxK
M3 - Article
C2 - 7596169
AN - SCOPUS:0029036446
VL - 9
SP - 1056
EP - 1059
JO - Leukemia
JF - Leukemia
SN - 0887-6924
IS - 6
ER -