Imaging of Rab5 activity identifies essential regulators for phagosome maturation

Masahiro Kitano, Nakaya Michio, Takeshi Nakamura, Shigekazu Nagata, Michiyuki Matsuda

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Efficient phagocytosis of apoptotic cells is crucial for tissue homeostasis and the immune response. Rab5 is known as a key regulator of the early endocytic pathway and we have recently shown that Rab5 is also implicated in apoptotic cell engulfment; however, the precise spatio-temporal dynamics of Rab5 activity remain unknown. Here, using a newly developed fluorescence resonance energy transfer biosensor, we describe a change in Rab5 activity during the engulfment of apoptotic thymocytes. Rab5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. Rab5 activation was either continuous or repetitive for up to 10 min, but it ended before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of Rab5 delayed this collapse of apoptotic thymocytes, showing a role for Rab5 in phagosome maturation. Disruption of microtubules with nocodazole inhibited Rab5 activation on the phagosome membrane without perturbing the engulfment of apoptotic cells. Furthermore, we found that Gapex-5 is the guanine nucleotide exchange factor essential for Rab5 activation during the engulfment of apoptotic cells. Gapex-5 was bound to a microtubule-tip- associating protein, EB1, whose depletion inhibited Rab5 activation during phagocytosis. We therefore propose a mechanistic model in which the recruitment of Gapex-5 to phagosomes through the microtubule network induces the transient Rab5 activation.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalNature
Volume453
Issue number7192
DOIs
Publication statusPublished - May 8 2008
Externally publishedYes

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Phagosomes
Microtubules
Thymocytes
Nocodazole
Guanine Nucleotide Exchange Factors
Cytophagocytosis
Fluorescence Resonance Energy Transfer
Membranes
Biosensing Techniques
Phagocytosis
Actins
Homeostasis
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Imaging of Rab5 activity identifies essential regulators for phagosome maturation. / Kitano, Masahiro; Michio, Nakaya; Nakamura, Takeshi; Nagata, Shigekazu; Matsuda, Michiyuki.

In: Nature, Vol. 453, No. 7192, 08.05.2008, p. 241-245.

Research output: Contribution to journalArticle

Kitano, M, Michio, N, Nakamura, T, Nagata, S & Matsuda, M 2008, 'Imaging of Rab5 activity identifies essential regulators for phagosome maturation', Nature, vol. 453, no. 7192, pp. 241-245. https://doi.org/10.1038/nature06857
Kitano, Masahiro ; Michio, Nakaya ; Nakamura, Takeshi ; Nagata, Shigekazu ; Matsuda, Michiyuki. / Imaging of Rab5 activity identifies essential regulators for phagosome maturation. In: Nature. 2008 ; Vol. 453, No. 7192. pp. 241-245.
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