Imatinib inhibits migration of normal human epidermal melanocytes and suppress the phosphorylation of C-kit

Jian xin Xia, Fu qiu Li, Kazunori Urabe, Jin feng Wang, Masutaka Furue

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To investigate the inhibitory effect of imatinib on the migration of normal human epidermal melanocytes (NHEM), the growth and differentiation of NHEM were measured by MTT assay. Transwell Cell Migration Assay was performed to determine the migration of NHEM following imatinib treatment. The phosphorylation of c-kit in NHEM after imatinib treatment was measured by western blot assay. Imatinib at 0.1 μM, 1.0 μM and 5.0 μM could inhibit the SCF (50 ng/mL) induced NHEM migration and suppress the phosphorylation of c-kit in NHEM. The result concluded that imatinib can inhibit SCF induced NHEM migration and suppress the c-kit phosphorylation in NHEM. This provide theoretical basis for the prevention of post-treatment recurrence of nevus spilus.

Original languageEnglish
Pages (from-to)100-103
Number of pages4
JournalBangladesh Journal of Pharmacology
Volume7
Issue number2
DOIs
Publication statusPublished - Jul 18 2012

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Melanocytes
Phosphorylation
Cell Migration Assays
Imatinib Mesylate
Nevus
Western Blotting
Recurrence
Growth

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Imatinib inhibits migration of normal human epidermal melanocytes and suppress the phosphorylation of C-kit. / Xia, Jian xin; Li, Fu qiu; Urabe, Kazunori; Wang, Jin feng; Furue, Masutaka.

In: Bangladesh Journal of Pharmacology, Vol. 7, No. 2, 18.07.2012, p. 100-103.

Research output: Contribution to journalArticle

Xia, Jian xin ; Li, Fu qiu ; Urabe, Kazunori ; Wang, Jin feng ; Furue, Masutaka. / Imatinib inhibits migration of normal human epidermal melanocytes and suppress the phosphorylation of C-kit. In: Bangladesh Journal of Pharmacology. 2012 ; Vol. 7, No. 2. pp. 100-103.
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