Imatinib inhibits migration of normal human epidermal melanocytes and suppress the phosphorylation of C-kit

Jian xin Xia; Fu qiu Li; Kazunori Urabe; Jin feng Wang; Masutaka Furue

doi:10.3329/bjp.v7i2.10800

Imatinib inhibits migration of normal human epidermal melanocytes and suppress the phosphorylation of C-kit

Jian xin Xia, Fu qiu Li, Kazunori Urabe, Jin feng Wang, Masutaka Furue

Surgery

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Abstract

To investigate the inhibitory effect of imatinib on the migration of normal human epidermal melanocytes (NHEM), the growth and differentiation of NHEM were measured by MTT assay. Transwell Cell Migration Assay was performed to determine the migration of NHEM following imatinib treatment. The phosphorylation of c-kit in NHEM after imatinib treatment was measured by western blot assay. Imatinib at 0.1 μM, 1.0 μM and 5.0 μM could inhibit the SCF (50 ng/mL) induced NHEM migration and suppress the phosphorylation of c-kit in NHEM. The result concluded that imatinib can inhibit SCF induced NHEM migration and suppress the c-kit phosphorylation in NHEM. This provide theoretical basis for the prevention of post-treatment recurrence of nevus spilus.

Original language	English
Pages (from-to)	100-103
Number of pages	4
Journal	Bangladesh Journal of Pharmacology
Volume	7
Issue number	2
DOIs	https://doi.org/10.3329/bjp.v7i2.10800
Publication status	Published - 2012

All Science Journal Classification (ASJC) codes

Pharmacology

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10.3329/bjp.v7i2.10800

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title = "Imatinib inhibits migration of normal human epidermal melanocytes and suppress the phosphorylation of C-kit",

abstract = "To investigate the inhibitory effect of imatinib on the migration of normal human epidermal melanocytes (NHEM), the growth and differentiation of NHEM were measured by MTT assay. Transwell Cell Migration Assay was performed to determine the migration of NHEM following imatinib treatment. The phosphorylation of c-kit in NHEM after imatinib treatment was measured by western blot assay. Imatinib at 0.1 μM, 1.0 μM and 5.0 μM could inhibit the SCF (50 ng/mL) induced NHEM migration and suppress the phosphorylation of c-kit in NHEM. The result concluded that imatinib can inhibit SCF induced NHEM migration and suppress the c-kit phosphorylation in NHEM. This provide theoretical basis for the prevention of post-treatment recurrence of nevus spilus.",

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T1 - Imatinib inhibits migration of normal human epidermal melanocytes and suppress the phosphorylation of C-kit

AU - Xia, Jian xin

AU - Li, Fu qiu

AU - Urabe, Kazunori

AU - Wang, Jin feng

AU - Furue, Masutaka

PY - 2012

Y1 - 2012

N2 - To investigate the inhibitory effect of imatinib on the migration of normal human epidermal melanocytes (NHEM), the growth and differentiation of NHEM were measured by MTT assay. Transwell Cell Migration Assay was performed to determine the migration of NHEM following imatinib treatment. The phosphorylation of c-kit in NHEM after imatinib treatment was measured by western blot assay. Imatinib at 0.1 μM, 1.0 μM and 5.0 μM could inhibit the SCF (50 ng/mL) induced NHEM migration and suppress the phosphorylation of c-kit in NHEM. The result concluded that imatinib can inhibit SCF induced NHEM migration and suppress the c-kit phosphorylation in NHEM. This provide theoretical basis for the prevention of post-treatment recurrence of nevus spilus.

AB - To investigate the inhibitory effect of imatinib on the migration of normal human epidermal melanocytes (NHEM), the growth and differentiation of NHEM were measured by MTT assay. Transwell Cell Migration Assay was performed to determine the migration of NHEM following imatinib treatment. The phosphorylation of c-kit in NHEM after imatinib treatment was measured by western blot assay. Imatinib at 0.1 μM, 1.0 μM and 5.0 μM could inhibit the SCF (50 ng/mL) induced NHEM migration and suppress the phosphorylation of c-kit in NHEM. The result concluded that imatinib can inhibit SCF induced NHEM migration and suppress the c-kit phosphorylation in NHEM. This provide theoretical basis for the prevention of post-treatment recurrence of nevus spilus.

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Imatinib inhibits migration of normal human epidermal melanocytes and suppress the phosphorylation of C-kit

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