Immediate-early gene expression in the brain of the thiamine-deficient rat

Alan S. Hazell, Lynda McGahan, Wolfram Tetzlaff, Annie M. Bedard, George S. Robertson, Yusaku Nakabeppu, Antoine M. Hakim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pyrithiamine-induced thiamine deficiency (PTD) in the rat is associated with neuronal loss in the thalamus and inferior colliculus. Recently, we were able to demonstrate the occurrence of apoptosis in the thalamus of these animals. Given that immediate-early genes (IEGs) participate in signal transduction pathways that mediate programmed cell death, the present study utilized in situ hybridization and immunohistochemistry to examine the expression of four IEGs (c-fos, c-jun, fos-B, and NGFI-A) during the progression of PTD. Elevated c-fos mRNA levels were initially observed in the posterior medial thalamus on d 12 of the deficiency. At the acute symptomatic stage (characterized by a loss of righting reflex on d 16-17), the posterior- medial thalamus exhibited increased mRNA for all genes examined, whereas the inferior colliculus demonstrated mRNA induction for c-fos, c-jun, and NGFI- A. Immunohistochemical analysis revealed that elevations of IEG mRNA associated with the acute symptomatic stage were consistently translated into protein in the thalamus. In contrast, whereas elevated Fos- and Jun-like immunoreactivity were detected in the inferior colliculus at this stage, NGFI-A-like immunoreactivity declined significantly below basal levels, suggesting a translational block. These results are consistent with our recent findings of apoptotic cell death, and indicate that differential patterns of IEG expression occur in the thalamus and inferior colliculus during PTD, which may contribute to the pathogenesis of this disorder.

Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalJournal of Molecular Neuroscience
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Immediate-Early Genes
Thiamine
Thalamus
Inferior Colliculi
Pyrithiamine
Thiamine Deficiency
Gene Expression
Brain
Messenger RNA
Cell Death
Righting Reflex
In Situ Hybridization
Signal Transduction
Immunohistochemistry
Apoptosis
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

Hazell, A. S., McGahan, L., Tetzlaff, W., Bedard, A. M., Robertson, G. S., Nakabeppu, Y., & Hakim, A. M. (1998). Immediate-early gene expression in the brain of the thiamine-deficient rat. Journal of Molecular Neuroscience, 10(1), 1-15. https://doi.org/10.1007/BF02737081

Immediate-early gene expression in the brain of the thiamine-deficient rat. / Hazell, Alan S.; McGahan, Lynda; Tetzlaff, Wolfram; Bedard, Annie M.; Robertson, George S.; Nakabeppu, Yusaku; Hakim, Antoine M.

In: Journal of Molecular Neuroscience, Vol. 10, No. 1, 01.01.1998, p. 1-15.

Research output: Contribution to journalArticle

Hazell, AS, McGahan, L, Tetzlaff, W, Bedard, AM, Robertson, GS, Nakabeppu, Y & Hakim, AM 1998, 'Immediate-early gene expression in the brain of the thiamine-deficient rat', Journal of Molecular Neuroscience, vol. 10, no. 1, pp. 1-15. https://doi.org/10.1007/BF02737081
Hazell, Alan S. ; McGahan, Lynda ; Tetzlaff, Wolfram ; Bedard, Annie M. ; Robertson, George S. ; Nakabeppu, Yusaku ; Hakim, Antoine M. / Immediate-early gene expression in the brain of the thiamine-deficient rat. In: Journal of Molecular Neuroscience. 1998 ; Vol. 10, No. 1. pp. 1-15.
@article{3d99c3ca4f4d4977aea5d39a3fa194f1,
title = "Immediate-early gene expression in the brain of the thiamine-deficient rat",
abstract = "Pyrithiamine-induced thiamine deficiency (PTD) in the rat is associated with neuronal loss in the thalamus and inferior colliculus. Recently, we were able to demonstrate the occurrence of apoptosis in the thalamus of these animals. Given that immediate-early genes (IEGs) participate in signal transduction pathways that mediate programmed cell death, the present study utilized in situ hybridization and immunohistochemistry to examine the expression of four IEGs (c-fos, c-jun, fos-B, and NGFI-A) during the progression of PTD. Elevated c-fos mRNA levels were initially observed in the posterior medial thalamus on d 12 of the deficiency. At the acute symptomatic stage (characterized by a loss of righting reflex on d 16-17), the posterior- medial thalamus exhibited increased mRNA for all genes examined, whereas the inferior colliculus demonstrated mRNA induction for c-fos, c-jun, and NGFI- A. Immunohistochemical analysis revealed that elevations of IEG mRNA associated with the acute symptomatic stage were consistently translated into protein in the thalamus. In contrast, whereas elevated Fos- and Jun-like immunoreactivity were detected in the inferior colliculus at this stage, NGFI-A-like immunoreactivity declined significantly below basal levels, suggesting a translational block. These results are consistent with our recent findings of apoptotic cell death, and indicate that differential patterns of IEG expression occur in the thalamus and inferior colliculus during PTD, which may contribute to the pathogenesis of this disorder.",
author = "Hazell, {Alan S.} and Lynda McGahan and Wolfram Tetzlaff and Bedard, {Annie M.} and Robertson, {George S.} and Yusaku Nakabeppu and Hakim, {Antoine M.}",
year = "1998",
month = "1",
day = "1",
doi = "10.1007/BF02737081",
language = "English",
volume = "10",
pages = "1--15",
journal = "Journal of Molecular Neuroscience",
issn = "0895-8696",
publisher = "Humana Press",
number = "1",

}

TY - JOUR

T1 - Immediate-early gene expression in the brain of the thiamine-deficient rat

AU - Hazell, Alan S.

AU - McGahan, Lynda

AU - Tetzlaff, Wolfram

AU - Bedard, Annie M.

AU - Robertson, George S.

AU - Nakabeppu, Yusaku

AU - Hakim, Antoine M.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Pyrithiamine-induced thiamine deficiency (PTD) in the rat is associated with neuronal loss in the thalamus and inferior colliculus. Recently, we were able to demonstrate the occurrence of apoptosis in the thalamus of these animals. Given that immediate-early genes (IEGs) participate in signal transduction pathways that mediate programmed cell death, the present study utilized in situ hybridization and immunohistochemistry to examine the expression of four IEGs (c-fos, c-jun, fos-B, and NGFI-A) during the progression of PTD. Elevated c-fos mRNA levels were initially observed in the posterior medial thalamus on d 12 of the deficiency. At the acute symptomatic stage (characterized by a loss of righting reflex on d 16-17), the posterior- medial thalamus exhibited increased mRNA for all genes examined, whereas the inferior colliculus demonstrated mRNA induction for c-fos, c-jun, and NGFI- A. Immunohistochemical analysis revealed that elevations of IEG mRNA associated with the acute symptomatic stage were consistently translated into protein in the thalamus. In contrast, whereas elevated Fos- and Jun-like immunoreactivity were detected in the inferior colliculus at this stage, NGFI-A-like immunoreactivity declined significantly below basal levels, suggesting a translational block. These results are consistent with our recent findings of apoptotic cell death, and indicate that differential patterns of IEG expression occur in the thalamus and inferior colliculus during PTD, which may contribute to the pathogenesis of this disorder.

AB - Pyrithiamine-induced thiamine deficiency (PTD) in the rat is associated with neuronal loss in the thalamus and inferior colliculus. Recently, we were able to demonstrate the occurrence of apoptosis in the thalamus of these animals. Given that immediate-early genes (IEGs) participate in signal transduction pathways that mediate programmed cell death, the present study utilized in situ hybridization and immunohistochemistry to examine the expression of four IEGs (c-fos, c-jun, fos-B, and NGFI-A) during the progression of PTD. Elevated c-fos mRNA levels were initially observed in the posterior medial thalamus on d 12 of the deficiency. At the acute symptomatic stage (characterized by a loss of righting reflex on d 16-17), the posterior- medial thalamus exhibited increased mRNA for all genes examined, whereas the inferior colliculus demonstrated mRNA induction for c-fos, c-jun, and NGFI- A. Immunohistochemical analysis revealed that elevations of IEG mRNA associated with the acute symptomatic stage were consistently translated into protein in the thalamus. In contrast, whereas elevated Fos- and Jun-like immunoreactivity were detected in the inferior colliculus at this stage, NGFI-A-like immunoreactivity declined significantly below basal levels, suggesting a translational block. These results are consistent with our recent findings of apoptotic cell death, and indicate that differential patterns of IEG expression occur in the thalamus and inferior colliculus during PTD, which may contribute to the pathogenesis of this disorder.

UR - http://www.scopus.com/inward/record.url?scp=0031814084&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031814084&partnerID=8YFLogxK

U2 - 10.1007/BF02737081

DO - 10.1007/BF02737081

M3 - Article

C2 - 9589366

AN - SCOPUS:0031814084

VL - 10

SP - 1

EP - 15

JO - Journal of Molecular Neuroscience

JF - Journal of Molecular Neuroscience

SN - 0895-8696

IS - 1

ER -