Immobilization and activity of enzymes on stereocomplex platforms

Yuya Nagasaka, Hisao Matsuno, Kimio Kurita, Takeshi Serizawa

Research output: Contribution to conferencePaper

Abstract

Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method on polystyrene microplate, and β-D-galactosidase (β-Gal) was immobilized on the PMMA stereocomplex film. Then, p-nitrophenyl-β-D-galactopyranoside (PNPG) solutions were added into the β-Gal immobilized wells. The enzymatic activity was determined by following the absorption at 405 nm by the ester cleavage of PNPG. The observation was also carried out on cast it-PMMA films. Effects of film surfaces on the enzyme reaction were investigated. As a result, it was found that the enzymatic activity of the immobilized β-Gal on PMMA stereocomplex films was higher than that on cast it-PMMA films.

Original languageEnglish
Number of pages1
Publication statusPublished - Oct 19 2006
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

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Polymethyl methacrylates
Enzymes
Organic solvents
Polystyrenes
Esters

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Nagasaka, Y., Matsuno, H., Kurita, K., & Serizawa, T. (2006). Immobilization and activity of enzymes on stereocomplex platforms. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.

Immobilization and activity of enzymes on stereocomplex platforms. / Nagasaka, Yuya; Matsuno, Hisao; Kurita, Kimio; Serizawa, Takeshi.

2006. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.

Research output: Contribution to conferencePaper

Nagasaka, Y, Matsuno, H, Kurita, K & Serizawa, T 2006, 'Immobilization and activity of enzymes on stereocomplex platforms', Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan, 5/24/06 - 5/26/06.
Nagasaka Y, Matsuno H, Kurita K, Serizawa T. Immobilization and activity of enzymes on stereocomplex platforms. 2006. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.
Nagasaka, Yuya ; Matsuno, Hisao ; Kurita, Kimio ; Serizawa, Takeshi. / Immobilization and activity of enzymes on stereocomplex platforms. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.1 p.
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