Immobilization of alkaline phosphatase on magnetic particles by site-specific and covalent cross-linking catalyzed by microbial transglutaminase

Kousuke Moriyama, Kyunga Sung, Masahiro Goto, Noriho Kamiya

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23 Citations (Scopus)

Abstract

Bacterial alkaline phosphatase (BAP) was site-specifically and covalently immobilized on magnetic particles (MPs) using the enzymatic reaction of microbial transglutaminase (MTG). Immobilization efficiency was affected by the chemical surface treatment of MPs and immobilized BAP exhibited more than 90% of the initial activity after 10 rounds of recycling.

Original languageEnglish
Pages (from-to)650-653
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume111
Issue number6
DOIs
Publication statusPublished - Jun 1 2011

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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