Immobilization of calcium and phosphate ions improves the osteoconductivity of titanium implants

Sunarso, Riki Toita, Kanji Tsuru, Kunio Ishikawa

Research output: Contribution to journalArticle

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Abstract

In this work, to elevate weak osteoconductivity of titanium (Ti) implant, we prepared a Ti implant having both calcium and phosphate ions on its surface. To modify calcium and phosphate ions onto Ti, phosphate ions were first immobilized by treating the Ti with a NaH2PO4 solution, followed by CaCl2 treatment to immobilize calcium ions, which created the calcium and phosphate ions-modified Ti (Ca-P-Ti). X-ray photoelectron spectroscopy and thin-layer X-ray diffraction measurement confirmed that both phosphate and calcium ions were co-immobilized onto the Ti surface on the molecular level. Three-hour after seeding MC3T3-E1 murine pre-osteoblast cells on substrates, cell number on Ca-P-Ti was much larger than that of Ti and phosphate-modified Ti (P-Ti), but was similar to that of calcium-modified Ti (Ca-Ti). Also, MC3T3-E1 cells on Ca-P-Ti expressed larger amount of vinculin, a focal adhesion protein, than those on other substrates, probably resulting in larger cell size as well as greater cell proliferation on Ca-P-Ti than those on other substrates. Alkaline phosphatase activity of cells on Ca-P-Ti was greater than those on Ti and P-Ti, but was almost comparable to that of Ca-Ti. Moreover, the largest amount of bone-like nodule formation was observed on Ca-P-Ti. These results provide evidence that calcium and phosphate ions-co-immobilization onto Ti increased the osteoconductivity of Ti by stimulating the responses of pre-osteoblast cells. This simple modification would be promising technique for bone tissue implant including dental and orthopedic implants.

Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalMaterials Science and Engineering C
Volume68
DOIs
Publication statusPublished - Nov 1 2016

Fingerprint

Titanium
immobilization
calcium
Calcium
phosphates
Phosphates
titanium
Ions
ions
Osteoblasts
osteoblasts
cells
Bone
calcium phosphate
Substrates
bones
Vinculin
Dental prostheses
Orthopedics
Cell proliferation

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Immobilization of calcium and phosphate ions improves the osteoconductivity of titanium implants. / Sunarso; Toita, Riki; Tsuru, Kanji; Ishikawa, Kunio.

In: Materials Science and Engineering C, Vol. 68, 01.11.2016, p. 291-298.

Research output: Contribution to journalArticle

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