Immobilization of surfactant-lipase complexes and their high heat resistance in organic media

Muneharu Goto, Chiaki Hatanaka, Masahiro Goto

    Research output: Contribution to journalArticlepeer-review

    20 Citations (Scopus)

    Abstract

    Surfactant-lipase complexes were immobilized in an n-vinyl-2-pyrrolidone gel matrix. Features of a native lipase and gel-immobilized surfactant-lipase complexes were measured by esterification reaction between lauric acid and benzyl alcohol in isooctane. Optimal gel-immobilized surfactant-lipase complex activity was 37.2 mol h-1 kg-1-lipase. Gel-immobilized lipase complexes showed a 51-fold increase in activity and exhibited superior heat resistance compared to native lipases. The optimum temperature for the immobilized lipase complex was 60°C, as compared to 37°C for native lipase activity. Gel-immobilized lipase complexes could be readily recovered, and their high activity was completely preserved, even after 10 reuses.

    Original languageEnglish
    Pages (from-to)91-94
    Number of pages4
    JournalBiochemical Engineering Journal
    Volume24
    Issue number1
    DOIs
    Publication statusPublished - May 15 2005

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Environmental Engineering
    • Bioengineering
    • Biomedical Engineering

    Fingerprint

    Dive into the research topics of 'Immobilization of surfactant-lipase complexes and their high heat resistance in organic media'. Together they form a unique fingerprint.

    Cite this