Immobilization of surfactant-lipase complexes and their high heat resistance in organic media

Muneharu Goto, Chiaki Hatanaka, Masahiro Goto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Surfactant-lipase complexes were immobilized in an n-vinyl-2-pyrrolidone gel matrix. Features of a native lipase and gel-immobilized surfactant-lipase complexes were measured by esterification reaction between lauric acid and benzyl alcohol in isooctane. Optimal gel-immobilized surfactant-lipase complex activity was 37.2 mol h-1 kg-1-lipase. Gel-immobilized lipase complexes showed a 51-fold increase in activity and exhibited superior heat resistance compared to native lipases. The optimum temperature for the immobilized lipase complex was 60°C, as compared to 37°C for native lipase activity. Gel-immobilized lipase complexes could be readily recovered, and their high activity was completely preserved, even after 10 reuses.

Original languageEnglish
Pages (from-to)91-94
Number of pages4
JournalBiochemical Engineering Journal
Volume24
Issue number1
DOIs
Publication statusPublished - May 15 2005

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Lipases
Lipase
Heat resistance
Surface-Active Agents
Immobilization
Surface active agents
Hot Temperature
Gels
lauric acid
Benzyl Alcohol
Esterification
Alcohols
Temperature
Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

Cite this

Immobilization of surfactant-lipase complexes and their high heat resistance in organic media. / Goto, Muneharu; Hatanaka, Chiaki; Goto, Masahiro.

In: Biochemical Engineering Journal, Vol. 24, No. 1, 15.05.2005, p. 91-94.

Research output: Contribution to journalArticle

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