Immobilized gellan sulfate surface for cell adhesion and multiplication: Development of cell-hybrid biomaterials using self-produced fibronectin

Keiichi Miyamoto, Akiko Kanemoto, Kenichi Hashimoto, Masayuki Tokita, Takashi Komai

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A new concept for cell-hybrid biomaterial is proposed in which human unbilical vein endothelial cells (HUVEC) are adhered to an immobilized gellan sulfate (GS) surface. Extra domain A containing fibronectin (EDA(+)FN) released from HUVEC is necessary for cell adhesion and multiplication. The material design in this study is based on these self-released cell adhesion proteins. The interaction between GS and EDA(+)FN was evaluated using the affinity constant (KA); the value obtained was 1.03×108 (M-1). These results suggest that the adhesion of HUVEC to GS may be supported by the adhesion of EDA(+)FN to GS. We also found that this new material adheres to HUVEC, allowing the reintroduction of EDA(+)FN, which is self-produced by the cell. This material is relatively easy to produce, not requiring the usual coating of adhesion proteins in pretreatment.

Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalInternational Journal of Biological Macromolecules
Volume30
Issue number2
DOIs
Publication statusPublished - Apr 8 2002

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

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