Immobilized-OPG-Fc on a titanium surface inhibits RANKL-dependent osteoclast differentiation in vitro

Seicho Makihira, Yuichi Mine, Hiroki Nikawa, Takahiro Shuto, Eduardo Kosaka, Masaru Sugiyama, Ryuji Hosokawa

Research output: Contribution to journalArticle

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Abstract

The purpose of the present study was to examine the effect of osteoprotegerin (OPG)-Fc fusion protein immobilized on a titanium surface on the initial differentiation of osteoclast precursor RAW264.7 cells. These cells were cultured on titanium specimens over which OPG-Fc was immobilized. The enhancement of tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA expression in RAW264.7 cells exposed to receptor activator of NF-κB ligand (RANKL) stimulation on OPG-Fc-coated titanium was significantly lower than that in RAW264.7 cells exposed to RANKL on titanium specimens without immobilized OPG-Fc (ANOVA, P < 0.01). Preincubation of OPG-Fc-coated titanium, in a medium supplemented with 10% fetal bovine serum at 37°C for two days before the cells were seeded, had no significant effect on the decrease in mRNA expression (ANOVA, P < 0.01). Taken together, these results indicate that OPG-Fc immobilized on a titanium surface blocks the differentiation of RAW264.7 cells induced by RANKL stimulation.

Original languageEnglish
Pages (from-to)647-653
Number of pages7
JournalJournal of Materials Science: Materials in Medicine
Volume21
Issue number2
DOIs
Publication statusPublished - Feb 1 2010

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Osteoprotegerin
Osteoclasts
Titanium
Analysis of variance (ANOVA)
Analysis of Variance
Cathepsin K
Immobilized Proteins
Messenger RNA
Phosphatases
Acid Phosphatase
In Vitro Techniques
Cell Differentiation
Cultured Cells
Fusion reactions
Ligands
Proteins
Acids
Serum

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Immobilized-OPG-Fc on a titanium surface inhibits RANKL-dependent osteoclast differentiation in vitro. / Makihira, Seicho; Mine, Yuichi; Nikawa, Hiroki; Shuto, Takahiro; Kosaka, Eduardo; Sugiyama, Masaru; Hosokawa, Ryuji.

In: Journal of Materials Science: Materials in Medicine, Vol. 21, No. 2, 01.02.2010, p. 647-653.

Research output: Contribution to journalArticle

Makihira, Seicho ; Mine, Yuichi ; Nikawa, Hiroki ; Shuto, Takahiro ; Kosaka, Eduardo ; Sugiyama, Masaru ; Hosokawa, Ryuji. / Immobilized-OPG-Fc on a titanium surface inhibits RANKL-dependent osteoclast differentiation in vitro. In: Journal of Materials Science: Materials in Medicine. 2010 ; Vol. 21, No. 2. pp. 647-653.
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