Immortalization of human T lymphocytes by oncogenes

Fumiyoshi Okano; Hirofumi Tachibana; Koichi Akiyama; Sanetaka Shirahata; Hiroki Murakami

doi:10.1007/BF00749871

Immortalization of human T lymphocytes by oncogenes

Fumiyoshi Okano, Hirofumi Tachibana, Koichi Akiyama, Sanetaka Shirahata, Hiroki Murakami

Food Science and Biotechnology

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4 Citations (Scopus)

Abstract

Immortalized human T cell lines were established by cotransfecting c-Ha-ras and c-myc oncogenes to lymph node lymphocytes. The cell lines kept growing for 3 months after establishment without a decrease in growth rate. The cells did not require interleukin-2(IL-2) for their growth, but addition of IL-2 stimulated the growth of these cells. Flow cytometric analysis revealed that these cells were T cells expressing CD4 or CD8 antigens. A CD4 positive (CD4⁺) cell line produced IL-6, indicating that the cell line belongs to helper T cells. The CD8 positive (CD8⁺) cell line possessed cytotoxicity to tumor cells, indicating that the cell line were killer T cells. Both cell lines were able to proliferate in serum-free medium indefinitely.

Original language	English
Pages (from-to)	205-211
Number of pages	7
Journal	Cytotechnology
Volume	11
Issue number	3
DOIs	https://doi.org/10.1007/BF00749871
Publication status	Published - Jan 1 1993

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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10.1007/BF00749871

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title = "Immortalization of human T lymphocytes by oncogenes",

abstract = "Immortalized human T cell lines were established by cotransfecting c-Ha-ras and c-myc oncogenes to lymph node lymphocytes. The cell lines kept growing for 3 months after establishment without a decrease in growth rate. The cells did not require interleukin-2(IL-2) for their growth, but addition of IL-2 stimulated the growth of these cells. Flow cytometric analysis revealed that these cells were T cells expressing CD4 or CD8 antigens. A CD4 positive (CD4+) cell line produced IL-6, indicating that the cell line belongs to helper T cells. The CD8 positive (CD8+) cell line possessed cytotoxicity to tumor cells, indicating that the cell line were killer T cells. Both cell lines were able to proliferate in serum-free medium indefinitely.",

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AU - Okano, Fumiyoshi

AU - Tachibana, Hirofumi

AU - Akiyama, Koichi

AU - Shirahata, Sanetaka

AU - Murakami, Hiroki

PY - 1993/1/1

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N2 - Immortalized human T cell lines were established by cotransfecting c-Ha-ras and c-myc oncogenes to lymph node lymphocytes. The cell lines kept growing for 3 months after establishment without a decrease in growth rate. The cells did not require interleukin-2(IL-2) for their growth, but addition of IL-2 stimulated the growth of these cells. Flow cytometric analysis revealed that these cells were T cells expressing CD4 or CD8 antigens. A CD4 positive (CD4+) cell line produced IL-6, indicating that the cell line belongs to helper T cells. The CD8 positive (CD8+) cell line possessed cytotoxicity to tumor cells, indicating that the cell line were killer T cells. Both cell lines were able to proliferate in serum-free medium indefinitely.

AB - Immortalized human T cell lines were established by cotransfecting c-Ha-ras and c-myc oncogenes to lymph node lymphocytes. The cell lines kept growing for 3 months after establishment without a decrease in growth rate. The cells did not require interleukin-2(IL-2) for their growth, but addition of IL-2 stimulated the growth of these cells. Flow cytometric analysis revealed that these cells were T cells expressing CD4 or CD8 antigens. A CD4 positive (CD4+) cell line produced IL-6, indicating that the cell line belongs to helper T cells. The CD8 positive (CD8+) cell line possessed cytotoxicity to tumor cells, indicating that the cell line were killer T cells. Both cell lines were able to proliferate in serum-free medium indefinitely.

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Immortalization of human T lymphocytes by oncogenes

Abstract

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