Immortalization of human T lymphocytes by oncogenes

Fumiyoshi Okano, Hirofumi Tachibana, Koichi Akiyama, Sanetaka Shirahata, Hiroki Murakami

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Immortalized human T cell lines were established by cotransfecting c-Ha-ras and c-myc oncogenes to lymph node lymphocytes. The cell lines kept growing for 3 months after establishment without a decrease in growth rate. The cells did not require interleukin-2(IL-2) for their growth, but addition of IL-2 stimulated the growth of these cells. Flow cytometric analysis revealed that these cells were T cells expressing CD4 or CD8 antigens. A CD4 positive (CD4+) cell line produced IL-6, indicating that the cell line belongs to helper T cells. The CD8 positive (CD8+) cell line possessed cytotoxicity to tumor cells, indicating that the cell line were killer T cells. Both cell lines were able to proliferate in serum-free medium indefinitely.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalCytotechnology
Volume11
Issue number3
DOIs
Publication statusPublished - Jan 1 1993

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T-cells
Oncogenes
Cells
T-Lymphocytes
Cell Line
Interleukin-2
Growth
CD8 Antigens
CD4 Antigens
myc Genes
Serum-Free Culture Media
Helper-Inducer T-Lymphocytes
Lymphocytes
Antigens
Cytotoxicity
Interleukin-6
Lymph Nodes
Tumors
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Okano, F., Tachibana, H., Akiyama, K., Shirahata, S., & Murakami, H. (1993). Immortalization of human T lymphocytes by oncogenes. Cytotechnology, 11(3), 205-211. https://doi.org/10.1007/BF00749871

Immortalization of human T lymphocytes by oncogenes. / Okano, Fumiyoshi; Tachibana, Hirofumi; Akiyama, Koichi; Shirahata, Sanetaka; Murakami, Hiroki.

In: Cytotechnology, Vol. 11, No. 3, 01.01.1993, p. 205-211.

Research output: Contribution to journalArticle

Okano, F, Tachibana, H, Akiyama, K, Shirahata, S & Murakami, H 1993, 'Immortalization of human T lymphocytes by oncogenes', Cytotechnology, vol. 11, no. 3, pp. 205-211. https://doi.org/10.1007/BF00749871
Okano F, Tachibana H, Akiyama K, Shirahata S, Murakami H. Immortalization of human T lymphocytes by oncogenes. Cytotechnology. 1993 Jan 1;11(3):205-211. https://doi.org/10.1007/BF00749871
Okano, Fumiyoshi ; Tachibana, Hirofumi ; Akiyama, Koichi ; Shirahata, Sanetaka ; Murakami, Hiroki. / Immortalization of human T lymphocytes by oncogenes. In: Cytotechnology. 1993 ; Vol. 11, No. 3. pp. 205-211.
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