Immunization with heat shock protein 105-pulsed dendritic cells leads to tumor rejection in mice

Kazunori Yokomine, Tetsuya Nakatsura, Motozumi Minohara, Jun Ichi Kira, Tatsuko Kubo, Yutaka Sasaki, Yasuharu Nishimura

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16 Citations (Scopus)

Abstract

Recently, we reported that heat shock protein 105 (HSP105) DNA vaccination induced anti-tumor immunity. In this study, we set up a preclinical study to investigate the usefulness of dendritic cells (DCs) pulsed with mouse HSP105 as a whole protein for cancer immunotherapy in vivo. The recombinant HSP105 did not induce DC maturation, and the mice vaccinated with HSP105-pulsed BM-DCs were markedly prevented from the growth of subcutaneous tumors, accompanied with a massive infiltration of both CD4+ T cells and CD8+ T cells into the tumors. In depletion experiments, we proved that both CD4+ T cells and CD8+ T cells play a crucial role in anti-tumor immunity. Both CD4+ T cells and CD8+ T cells specific to HSP105 were induced by stimulation with HSP105-pulsed DCs. As a result, vaccination of mice with BM-DCs pulsed with HSP105 itself could elicit a stronger tumor rejection in comparison to DNA vaccination.

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume343
Issue number1
DOIs
Publication statusPublished - Apr 28 2006

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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