TY - JOUR
T1 - Immunization with heat shock protein 105-pulsed dendritic cells leads to tumor rejection in mice
AU - Yokomine, Kazunori
AU - Nakatsura, Tetsuya
AU - Minohara, Motozumi
AU - Kira, Jun Ichi
AU - Kubo, Tatsuko
AU - Sasaki, Yutaka
AU - Nishimura, Yasuharu
N1 - Funding Information:
We thank Dr. Kyoichi Shimomura (Astellas Pharmaceutical Co.) for providing the cancer cell line. This work was supported in part by Grants-in-Aid (No. 12213111 for Y. Nishmura, and No. 14770142 for T. Nakatsura) from the Ministry of Education, Science, Technology, Sports and Culture, Japan, and The Sagawa Foundation for Promotion of Cancer Research and Meiji Institute of Health Science.
PY - 2006/4/28
Y1 - 2006/4/28
N2 - Recently, we reported that heat shock protein 105 (HSP105) DNA vaccination induced anti-tumor immunity. In this study, we set up a preclinical study to investigate the usefulness of dendritic cells (DCs) pulsed with mouse HSP105 as a whole protein for cancer immunotherapy in vivo. The recombinant HSP105 did not induce DC maturation, and the mice vaccinated with HSP105-pulsed BM-DCs were markedly prevented from the growth of subcutaneous tumors, accompanied with a massive infiltration of both CD4+ T cells and CD8+ T cells into the tumors. In depletion experiments, we proved that both CD4+ T cells and CD8+ T cells play a crucial role in anti-tumor immunity. Both CD4+ T cells and CD8+ T cells specific to HSP105 were induced by stimulation with HSP105-pulsed DCs. As a result, vaccination of mice with BM-DCs pulsed with HSP105 itself could elicit a stronger tumor rejection in comparison to DNA vaccination.
AB - Recently, we reported that heat shock protein 105 (HSP105) DNA vaccination induced anti-tumor immunity. In this study, we set up a preclinical study to investigate the usefulness of dendritic cells (DCs) pulsed with mouse HSP105 as a whole protein for cancer immunotherapy in vivo. The recombinant HSP105 did not induce DC maturation, and the mice vaccinated with HSP105-pulsed BM-DCs were markedly prevented from the growth of subcutaneous tumors, accompanied with a massive infiltration of both CD4+ T cells and CD8+ T cells into the tumors. In depletion experiments, we proved that both CD4+ T cells and CD8+ T cells play a crucial role in anti-tumor immunity. Both CD4+ T cells and CD8+ T cells specific to HSP105 were induced by stimulation with HSP105-pulsed DCs. As a result, vaccination of mice with BM-DCs pulsed with HSP105 itself could elicit a stronger tumor rejection in comparison to DNA vaccination.
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U2 - 10.1016/j.bbrc.2006.02.142
DO - 10.1016/j.bbrc.2006.02.142
M3 - Article
C2 - 16540092
AN - SCOPUS:33645100231
SN - 0006-291X
VL - 343
SP - 269
EP - 278
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -
