Immuno-chromatographic test-strip for skin cathepsin L analysis

M. Yamaguchi, S. Kosaka, Y. Tahara, A. Date

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The objective is to develop a methodology to analyze cathepsin L (Cat L) activity derived from the stratum corneum as an index of skin conditions. In this paper, a disposable type immuno-chromatographic test-strip (test-strip) is proposed for Cat L analysis. A fabricated test-strip for Cat L analysis consists of a sample pad, a conjugate pad, a nitrocellulose membrane and absorption pad. After dropping a sample, it was needed 90 s to reach the solution at the test-line on the test-strip. Three min after dropping, a reddish purple colored band was appeared as the test-line. The band visually observed clearly when the concentration of Cat L of the standard solution over 0.01 U/l. It was indicated a possibility of the disposable type immuno-chromatographic test-strip for the analysis of Cat L of skin.

Original languageEnglish
Title of host publicationECS Transactions - Chemical Sensors 8
Subtitle of host publicationChemical (Gas, Ion, Bio) Sensors and Analytical Systems
Pages67-73
Number of pages7
Edition11
DOIs
Publication statusPublished - 2008
Externally publishedYes
EventChemical Sensors 8: Chemical (Gas, Ion, Bio) Sensors and Analytical Systems - 214th ECS Meeting - Honolulu, HI, United States
Duration: Oct 12 2008Oct 17 2008

Publication series

NameECS Transactions
Number11
Volume16
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Other

OtherChemical Sensors 8: Chemical (Gas, Ion, Bio) Sensors and Analytical Systems - 214th ECS Meeting
CountryUnited States
CityHonolulu, HI
Period10/12/0810/17/08

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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