Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application

H. Yanagihara, R. Sakata, H. Minami, H. Tanaka, Y. Shoyama, H. Murakami

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Abstract

Immunoaffinity column chromatography using anti-forskolin monoclonal antibodies appears to be far superior to previously published separation methods based on crystallization and chromatography. The capacity of the immunoaffinity column as determined by ELISA is 9 μg ml-1. Forskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalAnalytica Chimica Acta
Volume335
Issue number1-2
DOIs
Publication statusPublished - Dec 10 1996

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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