Anti-solamargine and anti-ginsenoside Rb1 monoclonal antibodies were used for preparation of an immunoaffinity column. Total solasodine glycosides were separated directly from the crude extract of Solanum khasianum fruit, by the established immunoaffinity column. This method was specific for solasodine glycosides, which was detected by thin layer chromatography and eastern blotting. An immunoaffinity column, using antiginsenoside Rb1 monoclonal antibody, has made possible a single-step separation of ginsenoside Rb1 from a crude extract of ginseng roots (Panax ginseng).
|Number of pages||12|
|Journal||Journal of Liquid Chromatography and Related Technologies|
|Publication status||Published - Oct 10 2002|
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Pharmaceutical Science
- Clinical Biochemistry