Immunoaffinity column for isolation of bioactive compounds using monoclonal antibodies

Waraporn Putalun, Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

Anti-solamargine and anti-ginsenoside Rb1 monoclonal antibodies were used for preparation of an immunoaffinity column. Total solasodine glycosides were separated directly from the crude extract of Solanum khasianum fruit, by the established immunoaffinity column. This method was specific for solasodine glycosides, which was detected by thin layer chromatography and eastern blotting. An immunoaffinity column, using antiginsenoside Rb1 monoclonal antibody, has made possible a single-step separation of ginsenoside Rb1 from a crude extract of ginseng roots (Panax ginseng).

Original languageEnglish
Pages (from-to)2387-2398
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Volume25
Issue number13-15
DOIs
Publication statusPublished - Oct 10 2002

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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