Immunochemical detection of methamphetamine using polymer-protected ultrafine platinum particles

Emi Hifumi, Rie Kanmei, Masanori Ishimaru, Kosuke Shimizu, Michitaka Ohtaki, Taizo Uda

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Ultrafine platinum particles protected by poly (methyl acrylate-co-N- vinyl-2-pyrrolidone) were applied to the immunological detection of methamphetamine (MA). The polymer-protected ultrafine particles could chemically bind anti-methamphetamine monoclonal antibody to their surfaces. The antibody-fixed particles behaved like an antibody in the immunoreaction. The antibody-bound particles competitively reacted against the immobilized and the free antigens in the ELISA system. MA could be detected down to a concentration of ca. 10 ng/ml. In the immuno-dot assay, 10-100 ng/ml of MA was detected within 30 min using the ultrafine particles.

    Original languageEnglish
    Pages (from-to)417-419
    Number of pages3
    JournalJournal of Fermentation and Bioengineering
    Volume82
    Issue number4
    DOIs
    Publication statusPublished - 1996

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Applied Microbiology and Biotechnology

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