Immunochemical identification of the pssA gene product as phosphatidylserine synthase I of Chinese hamster ovary cells

Kyoko Saito, Osamu Kuge, Yuzuru Akamatsu, Masahiro Nishijima

Research output: Contribution to journalArticle

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Abstract

We have previously shown that a Chinese hamster ovary (CHO) cell mutant defective in phosphatidylserine synthase I recovers the enzyme activity on transfection with a pssA cDNA clone isolated from the parental CHO-K1. The resultant transfectant, CDT-1, exhibited about 20-fold higher specific activity of the enzyme in the membrane fraction than CHO-K1 cells. Polyclonal antibodies against two peptides of the predicted pssA product cross-reacted with a membrane protein having an apparent molecular mass of 42 kDa, which was overproduced in CDT-1 cells. By immunoprecipitation with the antibody, phosphatidylserine synthase I activity as web as the 42-kDa protein was eliminated from solubilized membrane proteins of CDT-1 cells. Both the enzyme activity and the 42-kDa protein of CHO-K1 cells were enriched in the mitochondria-associated membrane fraction and the microsome fraction, but neither was enriched in the mitochondria fraction or the cytosol fraction. These results suggest that the pssA gene encodes phosphatidylserine synthase I.

Original languageEnglish
Pages (from-to)262-266
Number of pages5
JournalFEBS Letters
Volume395
Issue number2-3
DOIs
Publication statusPublished - Oct 21 1996

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Cricetulus
Ovary
Mitochondria
Genes
Cells
Enzyme activity
Membrane Proteins
Membranes
Antibodies
Molecular mass
Enzymes
Proteins
Complementary DNA
Peptides
Microsomes
Immunoprecipitation
Cytosol
Transfection
Clone Cells
phospholipid serine base exchange enzyme

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Immunochemical identification of the pssA gene product as phosphatidylserine synthase I of Chinese hamster ovary cells. / Saito, Kyoko; Kuge, Osamu; Akamatsu, Yuzuru; Nishijima, Masahiro.

In: FEBS Letters, Vol. 395, No. 2-3, 21.10.1996, p. 262-266.

Research output: Contribution to journalArticle

Saito, Kyoko ; Kuge, Osamu ; Akamatsu, Yuzuru ; Nishijima, Masahiro. / Immunochemical identification of the pssA gene product as phosphatidylserine synthase I of Chinese hamster ovary cells. In: FEBS Letters. 1996 ; Vol. 395, No. 2-3. pp. 262-266.
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