TY - JOUR
T1 - Immunochromatographic determination of bacopaside I in biological samples
AU - Sookying, Sontaya
AU - Pekthong, Dumrongsak
AU - Oo-puthinan, Sarawut
AU - Phrompittayarat, Watoo
AU - Putalun, Waraporn
AU - Tanaka, Hiroyuki
AU - Xing, Jie
AU - Zhan, Zhaoqi
AU - Khorana, Nantaka
AU - Nuengchamnong, Nitra
AU - Ingkaninan, Kornkanok
N1 - Funding Information:
This research was supported by the Royal Golden Jubilee PhD Program , the Center of Excellence for Innovation in Chemistry , the Office of the Higher Education Commission and the Thailand Research Fund . The Customer Support Centre of Shimadzu (Asia Pacific) was thanked for providing LC–MS/MS in this study. We thanked Dr. Norman Scholfield for his help in manuscript preparation and Mrs. Charinrat Tothiam for her help on immunochromatographic technique.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - We describe a novel immunochromatographic method for qualitative and quantitative analyses of bacopaside I, a bioactive constituent in Bacopa monnieri (L.) Wettst in biological samples. The assay was performed on polyethersulfone membrane using a polyclonal antibody raised against bacopaside I. The finalised method could quantitatively determine bacopaside I in the range of 31.3-1000.0 ng and the detection and quantification limits were 1.0 and 31.3 ng, respectively. The percentage recoveries of bacopaside I in blood and urine were nearly 100% indicating the accuracy of the extraction. The method was then applied for the determination of this compound in rat serum, urine and feces after an oral dose of 15 mg/kg body weight. About 4% of the ingested dose of bacopaside I was detected in rat feces but none was detected in serum and urine which accorded with results from liquid chromatography tandem mass spectrometry. The accuracy, selectivity, sensitivity of the method are appropriate for in vivo pharmacokinetic studies.
AB - We describe a novel immunochromatographic method for qualitative and quantitative analyses of bacopaside I, a bioactive constituent in Bacopa monnieri (L.) Wettst in biological samples. The assay was performed on polyethersulfone membrane using a polyclonal antibody raised against bacopaside I. The finalised method could quantitatively determine bacopaside I in the range of 31.3-1000.0 ng and the detection and quantification limits were 1.0 and 31.3 ng, respectively. The percentage recoveries of bacopaside I in blood and urine were nearly 100% indicating the accuracy of the extraction. The method was then applied for the determination of this compound in rat serum, urine and feces after an oral dose of 15 mg/kg body weight. About 4% of the ingested dose of bacopaside I was detected in rat feces but none was detected in serum and urine which accorded with results from liquid chromatography tandem mass spectrometry. The accuracy, selectivity, sensitivity of the method are appropriate for in vivo pharmacokinetic studies.
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U2 - 10.1016/j.jchromb.2016.11.022
DO - 10.1016/j.jchromb.2016.11.022
M3 - Article
C2 - 27907870
AN - SCOPUS:84998865460
VL - 1040
SP - 60
EP - 66
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
ER -