Immunocytochemical localization of substance P neurokinin-1 receptors in rat gingival tissue

Mizuho A. Kido, Takayoshi Yamaza, Tetsuya Goto, Teruo Tanaka

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The distributions of substance P (SP) and the neurokinin-1 receptor (NK1-R), the receptor preferentially activated by SP, were examined in rat gingiva by immunocytochemical methods with light and electron microscopy. SP-immunoreactive nerve fibers were located preferentially in the junctional epithelium (JE) but few in the other oral and oral sulcular epithelia. NK1-R immunoreactivity was found in the endothelial cells (capillaries and postcapillary venules underlying the JE). NK1-R-labeled and -unlabeled unmyelinated nerve fibers were located close to the blood vessels and partially or completely covered by a Schwann cell sheath. In the JE, labeled naked axons without Schwann cell sheaths were observed. Neutrophils and macrophages in the connective tissue underlying the JE and in the JE were also labeled with NK1-R. Furthermore, NK1-R was found in the JE cells. Basically, immunoreaction products for NK1-R were found throughout various cells (endothelial cells, neutrophils, and JE cells) at invaginations of the plasma membrane and in vesicular and granular structures that are probably endosomes and are found close to both the plasma membrane and the nucleus. This is a first report, demonstrating the presence of NK1-R in the gingival tissue in the normal nonstimulated condition. Furthermore, it is thought that SP may modulate the permeability of blood vessels beneath the JE, the production of antimicrobial agents in neutrophils, and the proliferation and endocytotic ability of JE cells through NK1-R.

Original languageEnglish
Pages (from-to)213-222
Number of pages10
JournalCell and tissue research
Volume297
Issue number2
DOIs
Publication statusPublished - Aug 15 1999

Fingerprint

Epithelial Attachment
Neurokinin-1 Receptors
Substance P
Rats
Tissue
Neurilemma
Endothelial cells
Blood vessels
Cell membranes
Neutrophils
Schwann Cells
Cells
Blood Vessels
Fibers
Macrophages
Endothelial Cells
Anti-Infective Agents
Cell Membrane
Electron microscopy
Optical microscopy

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Immunocytochemical localization of substance P neurokinin-1 receptors in rat gingival tissue. / Kido, Mizuho A.; Yamaza, Takayoshi; Goto, Tetsuya; Tanaka, Teruo.

In: Cell and tissue research, Vol. 297, No. 2, 15.08.1999, p. 213-222.

Research output: Contribution to journalArticle

Kido, Mizuho A. ; Yamaza, Takayoshi ; Goto, Tetsuya ; Tanaka, Teruo. / Immunocytochemical localization of substance P neurokinin-1 receptors in rat gingival tissue. In: Cell and tissue research. 1999 ; Vol. 297, No. 2. pp. 213-222.
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