Immunocytochemical localization of the neurokinin 1 receptor in rat dental pulp

Mizuho A. Kido, Teiichi Ibuki, Atsushi Danjo, Teruyoshi Kondo, Jing Qi Zhang, Takayoshi Yamaza, Yoshio Yamashita, Yoshinori Higuchi, Teruo Tanaka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The dentin-pulp complex is a peripheral end-organ supplied by dense sensory nerve fibers. Substance P, a representative neuropeptide widely distributed in the dental pulp, has been reported to play roles in pain transmission and the amplification of inflammation. We analyzed here the expression of the neurokinin 1 (NK1) receptor, preferentially activated by substance P, using immunocytochemistry in rat dental pulp at both the light and electron microscopic levels. Conspicuous NK1 receptor immunoreactivity was found in the odontoblasts; immunolabelings were present at their plasma membrane and endosomal structures, especially in their cytoplasmic processes. Immunoreactions for NK1 receptor were also detectable in a part of the nerve terminals associated with the cytoplasmic processes of the odontoblasts. Furthermore, the endothelial cells of capillaries and post-capillary venules and the fibroblasts were labeled with the NK1 receptor in the subodontoblast layer. These findings suggest that pulpal cells and nerve fibers are targets for substance P that mediate multiple functions, including a vasoactive function and the regulation of vascular permeability as well as the modulation of pain transmission.

Original languageEnglish
Pages (from-to)259-265
Number of pages7
JournalArchives of Histology and Cytology
Volume68
Issue number4
DOIs
Publication statusPublished - Dec 1 2005

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Neurokinin-1 Receptors
Dental Pulp
Substance P
Odontoblasts
Nerve Fibers
Pain
Venules
Capillary Permeability
Dentin
Neuropeptides
Endothelial Cells
Fibroblasts
Immunohistochemistry
Cell Membrane
Electrons
Inflammation
Light

All Science Journal Classification (ASJC) codes

  • Histology

Cite this

Immunocytochemical localization of the neurokinin 1 receptor in rat dental pulp. / Kido, Mizuho A.; Ibuki, Teiichi; Danjo, Atsushi; Kondo, Teruyoshi; Zhang, Jing Qi; Yamaza, Takayoshi; Yamashita, Yoshio; Higuchi, Yoshinori; Tanaka, Teruo.

In: Archives of Histology and Cytology, Vol. 68, No. 4, 01.12.2005, p. 259-265.

Research output: Contribution to journalArticle

Kido, MA, Ibuki, T, Danjo, A, Kondo, T, Zhang, JQ, Yamaza, T, Yamashita, Y, Higuchi, Y & Tanaka, T 2005, 'Immunocytochemical localization of the neurokinin 1 receptor in rat dental pulp', Archives of Histology and Cytology, vol. 68, no. 4, pp. 259-265. https://doi.org/10.1679/aohc.68.259
Kido, Mizuho A. ; Ibuki, Teiichi ; Danjo, Atsushi ; Kondo, Teruyoshi ; Zhang, Jing Qi ; Yamaza, Takayoshi ; Yamashita, Yoshio ; Higuchi, Yoshinori ; Tanaka, Teruo. / Immunocytochemical localization of the neurokinin 1 receptor in rat dental pulp. In: Archives of Histology and Cytology. 2005 ; Vol. 68, No. 4. pp. 259-265.
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