Immunocytochemistry of types I-IV collagen in human anterior subcapsular cataracts

Tatsuhiko Hatae, Tatsuro Ishibashi, Fumiaki Yoshitomi, Yosaburo Shibata

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Human anterior subcapsular cataracts were examined by immunocytochemistry to investigate which types of collagen constitutes the subcapsular fibrous matrix. Types I, III, and IV collagen were shown to be immunologically positive by both light and electron microscopy. In light microscopy, types I and III collagen were stained diffusely whereas type IV collagen was stained in a streaky pattern in the opacities. The lens capsules were stained with antibody to type IV collagen, although the staining patterns were not homogeneous; the inner layers of the capsules were labeled more strongly than the outer layers. Neither type I nor III collagen was detected in the capsules. In electron microscopy, collagen fibrils were labeled with the antibodies to both type I and type III collagen. The lens capsules and multilamellae of the basement membranes in the opacities were labeled with the antibody to type IV collagen.

Original languageEnglish
Pages (from-to)586-590
Number of pages5
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume231
Issue number10
DOIs
Publication statusPublished - Oct 1 1993

Fingerprint

Collagen Type IV
Collagen Type I
Cataract
Capsules
Collagen Type III
Immunohistochemistry
Collagen
Lenses
Antibodies
Electron Microscopy
Light
Basement Membrane
Microscopy
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Immunocytochemistry of types I-IV collagen in human anterior subcapsular cataracts. / Hatae, Tatsuhiko; Ishibashi, Tatsuro; Yoshitomi, Fumiaki; Shibata, Yosaburo.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 231, No. 10, 01.10.1993, p. 586-590.

Research output: Contribution to journalArticle

@article{9a9d95ee6ec44587b6491f437fd4c5df,
title = "Immunocytochemistry of types I-IV collagen in human anterior subcapsular cataracts",
abstract = "Human anterior subcapsular cataracts were examined by immunocytochemistry to investigate which types of collagen constitutes the subcapsular fibrous matrix. Types I, III, and IV collagen were shown to be immunologically positive by both light and electron microscopy. In light microscopy, types I and III collagen were stained diffusely whereas type IV collagen was stained in a streaky pattern in the opacities. The lens capsules were stained with antibody to type IV collagen, although the staining patterns were not homogeneous; the inner layers of the capsules were labeled more strongly than the outer layers. Neither type I nor III collagen was detected in the capsules. In electron microscopy, collagen fibrils were labeled with the antibodies to both type I and type III collagen. The lens capsules and multilamellae of the basement membranes in the opacities were labeled with the antibody to type IV collagen.",
author = "Tatsuhiko Hatae and Tatsuro Ishibashi and Fumiaki Yoshitomi and Yosaburo Shibata",
year = "1993",
month = "10",
day = "1",
doi = "10.1007/BF00936523",
language = "English",
volume = "231",
pages = "586--590",
journal = "Albrecht von Graefes Archiv für Klinische und Experimentelle Ophthalmologie",
issn = "0065-6100",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - Immunocytochemistry of types I-IV collagen in human anterior subcapsular cataracts

AU - Hatae, Tatsuhiko

AU - Ishibashi, Tatsuro

AU - Yoshitomi, Fumiaki

AU - Shibata, Yosaburo

PY - 1993/10/1

Y1 - 1993/10/1

N2 - Human anterior subcapsular cataracts were examined by immunocytochemistry to investigate which types of collagen constitutes the subcapsular fibrous matrix. Types I, III, and IV collagen were shown to be immunologically positive by both light and electron microscopy. In light microscopy, types I and III collagen were stained diffusely whereas type IV collagen was stained in a streaky pattern in the opacities. The lens capsules were stained with antibody to type IV collagen, although the staining patterns were not homogeneous; the inner layers of the capsules were labeled more strongly than the outer layers. Neither type I nor III collagen was detected in the capsules. In electron microscopy, collagen fibrils were labeled with the antibodies to both type I and type III collagen. The lens capsules and multilamellae of the basement membranes in the opacities were labeled with the antibody to type IV collagen.

AB - Human anterior subcapsular cataracts were examined by immunocytochemistry to investigate which types of collagen constitutes the subcapsular fibrous matrix. Types I, III, and IV collagen were shown to be immunologically positive by both light and electron microscopy. In light microscopy, types I and III collagen were stained diffusely whereas type IV collagen was stained in a streaky pattern in the opacities. The lens capsules were stained with antibody to type IV collagen, although the staining patterns were not homogeneous; the inner layers of the capsules were labeled more strongly than the outer layers. Neither type I nor III collagen was detected in the capsules. In electron microscopy, collagen fibrils were labeled with the antibodies to both type I and type III collagen. The lens capsules and multilamellae of the basement membranes in the opacities were labeled with the antibody to type IV collagen.

UR - http://www.scopus.com/inward/record.url?scp=0027370137&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027370137&partnerID=8YFLogxK

U2 - 10.1007/BF00936523

DO - 10.1007/BF00936523

M3 - Article

VL - 231

SP - 586

EP - 590

JO - Albrecht von Graefes Archiv für Klinische und Experimentelle Ophthalmologie

JF - Albrecht von Graefes Archiv für Klinische und Experimentelle Ophthalmologie

SN - 0065-6100

IS - 10

ER -