Immunodetection of ginsenoside Rb1 in rat serum

Li ling Ma, Zhi Chao, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To establish a novel immunoassay for qualitative detection of ginsenoside Rb1 in rat serum. METHODS: Anti-G-Rb1 monoclonal antibody (mAb) was through a hybridoma approach. Rat serum containing G-Rb1 was deproteinized with methanol to prepare the sample for testing, which was loaded onto polyethersulfone (PES) membrane and developed in the mixture of acetonitrile, water and acetic acid (25:75:1). After treatment with NaIO(4), the membrane was transferred to 1% BSA solution for immobilization of G-Rb1. The membrane was subsequently treated with anti-G-Rb1 mAb solution, followed by addition of peroxidase-labeled goat anti-mouse IgG and color development using 4-chloro-1-naphthol-0.03% H(2)O(2). RESULTS: On the PES membrane, a clear blue spot representing G-Rb1 occurred where the rat serum for testing and the standard G-Rb1 samples were blotted. The limit of this immunodetection was 0.25 microg. CONCLUSION: This immunoassay has greater specificity and reliability than thin-layer chromatography with a sensitivity similar to that of high-performance liquid chromatography, and does not require sophisticated equipment for convenient G-Rb1 detection in rat serum.

Original languageEnglish
Pages (from-to)1915-1917
Number of pages3
JournalNan fang yi ke da xue xue bao = Journal of Southern Medical University
Volume27
Issue number12
Publication statusPublished - Jan 1 2007

Fingerprint

Membranes
Serum
Immunoassay
Monoclonal Antibodies
Hybridomas
Thin Layer Chromatography
Goats
Immobilization
Acetic Acid
Peroxidase
Methanol
Color
High Pressure Liquid Chromatography
Equipment and Supplies
ginsenoside Rb1
Water
polyether sulfone
anti-IgG
acetonitrile
Suby's G solution

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Ma, L. L., Chao, Z., Tanaka, H., & Shoyama, Y. (2007). Immunodetection of ginsenoside Rb1 in rat serum. Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 27(12), 1915-1917.

Immunodetection of ginsenoside Rb1 in rat serum. / Ma, Li ling; Chao, Zhi; Tanaka, Hiroyuki; Shoyama, Yukihiro.

In: Nan fang yi ke da xue xue bao = Journal of Southern Medical University, Vol. 27, No. 12, 01.01.2007, p. 1915-1917.

Research output: Contribution to journalArticle

Ma, LL, Chao, Z, Tanaka, H & Shoyama, Y 2007, 'Immunodetection of ginsenoside Rb1 in rat serum', Nan fang yi ke da xue xue bao = Journal of Southern Medical University, vol. 27, no. 12, pp. 1915-1917.
Ma, Li ling ; Chao, Zhi ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Immunodetection of ginsenoside Rb1 in rat serum. In: Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 2007 ; Vol. 27, No. 12. pp. 1915-1917.
@article{907313336a0d4c5f846cf01571e996c7,
title = "Immunodetection of ginsenoside Rb1 in rat serum",
abstract = "OBJECTIVE: To establish a novel immunoassay for qualitative detection of ginsenoside Rb1 in rat serum. METHODS: Anti-G-Rb1 monoclonal antibody (mAb) was through a hybridoma approach. Rat serum containing G-Rb1 was deproteinized with methanol to prepare the sample for testing, which was loaded onto polyethersulfone (PES) membrane and developed in the mixture of acetonitrile, water and acetic acid (25:75:1). After treatment with NaIO(4), the membrane was transferred to 1{\%} BSA solution for immobilization of G-Rb1. The membrane was subsequently treated with anti-G-Rb1 mAb solution, followed by addition of peroxidase-labeled goat anti-mouse IgG and color development using 4-chloro-1-naphthol-0.03{\%} H(2)O(2). RESULTS: On the PES membrane, a clear blue spot representing G-Rb1 occurred where the rat serum for testing and the standard G-Rb1 samples were blotted. The limit of this immunodetection was 0.25 microg. CONCLUSION: This immunoassay has greater specificity and reliability than thin-layer chromatography with a sensitivity similar to that of high-performance liquid chromatography, and does not require sophisticated equipment for convenient G-Rb1 detection in rat serum.",
author = "Ma, {Li ling} and Zhi Chao and Hiroyuki Tanaka and Yukihiro Shoyama",
year = "2007",
month = "1",
day = "1",
language = "English",
volume = "27",
pages = "1915--1917",
journal = "Di yi jun yi da xue xue bao = Academic journal of the First Medical College of PLA",
issn = "1673-4254",
publisher = "Nanfang Yi Ke Da Xue Xue Bao Bian ji Bu",
number = "12",

}

TY - JOUR

T1 - Immunodetection of ginsenoside Rb1 in rat serum

AU - Ma, Li ling

AU - Chao, Zhi

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

PY - 2007/1/1

Y1 - 2007/1/1

N2 - OBJECTIVE: To establish a novel immunoassay for qualitative detection of ginsenoside Rb1 in rat serum. METHODS: Anti-G-Rb1 monoclonal antibody (mAb) was through a hybridoma approach. Rat serum containing G-Rb1 was deproteinized with methanol to prepare the sample for testing, which was loaded onto polyethersulfone (PES) membrane and developed in the mixture of acetonitrile, water and acetic acid (25:75:1). After treatment with NaIO(4), the membrane was transferred to 1% BSA solution for immobilization of G-Rb1. The membrane was subsequently treated with anti-G-Rb1 mAb solution, followed by addition of peroxidase-labeled goat anti-mouse IgG and color development using 4-chloro-1-naphthol-0.03% H(2)O(2). RESULTS: On the PES membrane, a clear blue spot representing G-Rb1 occurred where the rat serum for testing and the standard G-Rb1 samples were blotted. The limit of this immunodetection was 0.25 microg. CONCLUSION: This immunoassay has greater specificity and reliability than thin-layer chromatography with a sensitivity similar to that of high-performance liquid chromatography, and does not require sophisticated equipment for convenient G-Rb1 detection in rat serum.

AB - OBJECTIVE: To establish a novel immunoassay for qualitative detection of ginsenoside Rb1 in rat serum. METHODS: Anti-G-Rb1 monoclonal antibody (mAb) was through a hybridoma approach. Rat serum containing G-Rb1 was deproteinized with methanol to prepare the sample for testing, which was loaded onto polyethersulfone (PES) membrane and developed in the mixture of acetonitrile, water and acetic acid (25:75:1). After treatment with NaIO(4), the membrane was transferred to 1% BSA solution for immobilization of G-Rb1. The membrane was subsequently treated with anti-G-Rb1 mAb solution, followed by addition of peroxidase-labeled goat anti-mouse IgG and color development using 4-chloro-1-naphthol-0.03% H(2)O(2). RESULTS: On the PES membrane, a clear blue spot representing G-Rb1 occurred where the rat serum for testing and the standard G-Rb1 samples were blotted. The limit of this immunodetection was 0.25 microg. CONCLUSION: This immunoassay has greater specificity and reliability than thin-layer chromatography with a sensitivity similar to that of high-performance liquid chromatography, and does not require sophisticated equipment for convenient G-Rb1 detection in rat serum.

UR - http://www.scopus.com/inward/record.url?scp=77953102832&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953102832&partnerID=8YFLogxK

M3 - Article

C2 - 18159019

AN - SCOPUS:77953102832

VL - 27

SP - 1915

EP - 1917

JO - Di yi jun yi da xue xue bao = Academic journal of the First Medical College of PLA

JF - Di yi jun yi da xue xue bao = Academic journal of the First Medical College of PLA

SN - 1673-4254

IS - 12

ER -