Immunodominance of conformation-dependent B-cell epitopes of protein antigens

Hiro O. Ito, Toshihiro Nakashima, Takanori So, Masato Hirata, Masakazu Inoue

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Immunodominance of conformational epitopes over linear ones in four proteins was quantified making use of the B-cell hybridoma technology. The proteins were immunized in their native forms into BALB/c mice, and clonal frequencies of B-cell hybridomas that produce antibodies to the native and denatured forms were determined, using ELISA and immunoblotting. All 16 monoclonal antibodies (mAbs) to Porphyromonas gingivalis fimbria were suggested to recognize conformational epitopes expressed by the oligomer. Ten out of 14 mAbs to Serratia marcescens fimbria and 13 of 15 mAbs to hen lysozyme were also specific to their conformational epitopes. In contrast, all 18 mAbs to a surface protein of Streptococcus mutans, termed PAc, reacted to both the native and denatured forms, thereby indicating the immunodominance of linear epitopes in this protein. The results suggest that B-cell epitopes of proteins possessing stable tertiary or quaternary structures are predominantly expressed by the higher-order structures.

Original languageEnglish
Pages (from-to)770-776
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume308
Issue number4
DOIs
Publication statusPublished - Sep 5 2003

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B-Lymphocyte Epitopes
Conformations
Epitopes
Monoclonal Antibodies
Antigens
Hybridomas
Proteins
B-Lymphocytes
Cells
Porphyromonas gingivalis
Serratia marcescens
Streptococcus mutans
Muramidase
Oligomers
Immunoblotting
Membrane Proteins
Enzyme-Linked Immunosorbent Assay
Technology
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Immunodominance of conformation-dependent B-cell epitopes of protein antigens. / Ito, Hiro O.; Nakashima, Toshihiro; So, Takanori; Hirata, Masato; Inoue, Masakazu.

In: Biochemical and Biophysical Research Communications, Vol. 308, No. 4, 05.09.2003, p. 770-776.

Research output: Contribution to journalArticle

Ito, Hiro O. ; Nakashima, Toshihiro ; So, Takanori ; Hirata, Masato ; Inoue, Masakazu. / Immunodominance of conformation-dependent B-cell epitopes of protein antigens. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 308, No. 4. pp. 770-776.
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