Immunoenzyme localization of cathepsins in the Golgi region of rat hepatocytes and renal tubule cells

S. Yokota, Y. Nishimura, T. Kawabata, K. Kato

Research output: Contribution to journalArticle

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Abstract

Localization of carboxyl proteinase (cathepsin D) and cysteine proteinases (cathepsins B, H, and L) in Golgi region was studied using an immunoenzyme technique. Rat livers and kidneys were used. The results obtained from the livers were similar to those from the kidneys. All cathepsins were detected in lysosomal compartments such as secondary lysosomes, multivesicular bodies (endosomes), and autophagosomes. Rough endoplasmic reticulum (rER), including nuclear envelope was focally stained. Most of Golgi cisternae were negative, but sometimes only one cisterna or the terminal portion of the cisterna were stained focally. Rarely, the trans Golgi network (TGN) was positive for the proteinases. Among numerous Golgi vesicles, only a few of them were stained. The positive vesicles were divided into two groups, one had a bristle coat and heavily stained, and other were smaller than 40 nm in diameter and weakly stained. The small vesicles seemed to bud from the ER and to fuse with the Golgi cisternae, while the large clathrin-coated vesicles seem to bud from the TGN. The results suggests that cathepsins are transported by vesicular system from the rER to lysosomes via Golgi apparatus. In addition, it is suggested that the small vesicles transport the proteinases from the ER to the Golgi cisternae and the large clathrin-coated vesicles from the Golgi cisternae to the lysosomes.

Original languageEnglish
Pages (from-to)629-635
Number of pages7
JournalHistochemistry
Volume94
Issue number6
DOIs
Publication statusPublished - Nov 1 1990

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cathepsins
Cathepsins
renal tubules
lysosomes
Lysosomes
Clathrin-Coated Vesicles
hepatocytes
trans-Golgi Network
coated vesicles
clathrin
Hepatocytes
proteinases
yeast proteinase B
Rough Endoplasmic Reticulum
rough endoplasmic reticulum
Kidney
rats
Peptide Hydrolases
Cathepsin H
buds

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Agricultural and Biological Sciences(all)

Cite this

Immunoenzyme localization of cathepsins in the Golgi region of rat hepatocytes and renal tubule cells. / Yokota, S.; Nishimura, Y.; Kawabata, T.; Kato, K.

In: Histochemistry, Vol. 94, No. 6, 01.11.1990, p. 629-635.

Research output: Contribution to journalArticle

Yokota, S. ; Nishimura, Y. ; Kawabata, T. ; Kato, K. / Immunoenzyme localization of cathepsins in the Golgi region of rat hepatocytes and renal tubule cells. In: Histochemistry. 1990 ; Vol. 94, No. 6. pp. 629-635.
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