Immunofluorescence localization of type I and type III collagen and fibronectin in mouse dental tissues in late development and during molar eruption

K. Takita, Yasuyoshi Ohsaki, M. Nakata, K. Kurisu

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Affinity-purified antibodies produced intense staining for type I collagen in alveolar bone matrix and predentine, and moderate staining in the dentine matrix, lamina propria, connective tissue invaginating into papillary layer of the enamel organ, dental sac and periodontal ligament. No staining occurred in oral epithelium, stellate reticulum, stratum intermedium, ameloblasts and odontoblasts. Fibronectin was distributed similarly except at the interface between the epithelial diaphragm and pre-odontoblasts where type I collagen was absent but fibronectin was present. In contrast, type III collagen showed strong staining in the periodontal ligament and lamina propria but no staining in bone matrix, predentine, dentine and at the interface between the epithelial diaphragm and pre-odontoblasts. The staining pattern for type III collagen was similar to that of type I and fibronectin in other tissues including endosteal reticular tissue, the connective tissue invaginating into papillary layer and the extracellular matrix of the pulp.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalArchives of Oral Biology
Volume32
Issue number4
DOIs
Publication statusPublished - Jan 1 1987

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Collagen Type III
Collagen Type I
Fibronectins
Fluorescent Antibody Technique
Tooth
Staining and Labeling
Odontoblasts
Periodontal Ligament
Bone Matrix
Dentin
Diaphragm
Connective Tissue
Mucous Membrane
Dental Sac
Enamel Organ
Ameloblasts
Reticulum
Antibody Affinity
Extracellular Matrix
Epithelium

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Immunofluorescence localization of type I and type III collagen and fibronectin in mouse dental tissues in late development and during molar eruption. / Takita, K.; Ohsaki, Yasuyoshi; Nakata, M.; Kurisu, K.

In: Archives of Oral Biology, Vol. 32, No. 4, 01.01.1987, p. 273-279.

Research output: Contribution to journalArticle

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