Immunogenetic analysis of silicosis in Japan.

K. Honda, A. Kimura, R. P. Dong, H. Tamai, H. Nagato, Y. Nishimura, T. Sasazuki

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Abstract

We previously reported that a gene in linkage disequilibrium with HLA-Bw54, DR4, and DRw53 might control the susceptibility to silicosis (K. Honda et al. 1988. N. Engl. J. Med. 319:1610). To further define the HLA-linked gene and other genetic factors for predisposition of silicosis, we determined for HLA-DQ and DP alleles using the polymerase chain reaction and sequence-specific oligonucleotide probes and made a restriction fragment length polymorphism (RFLP) analysis of the fourth component of complement (C4) genes, immunoglobulin lambda variable chain (IGLV) gene, and T-cell receptor alpha and beta genes in 46 Japanese patients with silicosis. The frequency of DQB1*0401 (relative risk [RR] = 2.2, P < 0.02) was increased and that of DQB1*0601 (RR = 0.36, P < 0.01) was decreased in the patients. RFLP analysis of C4 and IGLV genes showed significant association between silicosis and a specific RFLP pattern of C4A3-C4B5 allotype (RR = 2.3, P < 0.05) and that of IGLV 5.3 kb (RR = 0.33, P < 0.003). No other genetic markers showed significant association. Statistical analyses of the associated genetic markers revealed that the HLA-Bw54 was the allele that showed primary association with silicosis and the frequencies of the C4 and HLA-DQ alleles were suggested to be increased due to their linkage disequilibrium with the HLA-Bw54. We conclude that the major gene for silicosis may be mapped near the HLA-B locus.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 1993

Fingerprint

Immunogenetics
Silicosis
Immunoglobulin lambda-Chains
Japan
Genes
Restriction Fragment Length Polymorphisms
HLA-DQ Antigens
Polymorphism
Alleles
Immunoglobulins
Linkage Disequilibrium
Genetic Markers
T-Cell Receptor alpha Genes
HLA-DP Antigens
T-Cell Receptor beta Genes
HLA-DR4 Antigen
Complement C4
HLA-B Antigens
Oligonucleotide Probes
Antigen Receptors, T-Cell, alpha-beta

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Honda, K., Kimura, A., Dong, R. P., Tamai, H., Nagato, H., Nishimura, Y., & Sasazuki, T. (1993). Immunogenetic analysis of silicosis in Japan. American journal of respiratory cell and molecular biology, 8(1), 106-111. https://doi.org/10.1165/ajrcmb/8.1.106

Immunogenetic analysis of silicosis in Japan. / Honda, K.; Kimura, A.; Dong, R. P.; Tamai, H.; Nagato, H.; Nishimura, Y.; Sasazuki, T.

In: American journal of respiratory cell and molecular biology, Vol. 8, No. 1, 01.1993, p. 106-111.

Research output: Contribution to journalArticle

Honda, K, Kimura, A, Dong, RP, Tamai, H, Nagato, H, Nishimura, Y & Sasazuki, T 1993, 'Immunogenetic analysis of silicosis in Japan.', American journal of respiratory cell and molecular biology, vol. 8, no. 1, pp. 106-111. https://doi.org/10.1165/ajrcmb/8.1.106
Honda, K. ; Kimura, A. ; Dong, R. P. ; Tamai, H. ; Nagato, H. ; Nishimura, Y. ; Sasazuki, T. / Immunogenetic analysis of silicosis in Japan. In: American journal of respiratory cell and molecular biology. 1993 ; Vol. 8, No. 1. pp. 106-111.
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