Immunohistochemical and scanning electron microscopic comparison of the collagen network constructions between pig, goat and chicken livers

Shotaro Nishimura, Ayano Sagara, Ichiro Oshima, Yoshitaka Ono, Hisao Iwamoto, Kaoru Okano, Hideyuki Miyachi, Shoji Tabata

Research output: Contribution to journalArticle

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Abstract

The distribution and three-dimensional architecture of collagen fibers were compared between pig, goat and chicken livers. Immunohistochemical staining revealed that collagen type I was identified in the interlobular connective tissue region and intralobular areas in pigs and goats. Type III collagen was also identified in the interlobular connective tissue region and intralobular sinusoidal walls. In the chicken liver, only the circumference region of the vessels was immunostained with collagen type I and III antibodies and the interlobular connective tissue wall could not be distinguished clearly. In the intralobular region, collagen type I antibody immunoreacted around the hepatic cells but collagen type III antibody immunoreacted weakly. In the NaOH macerated specimen, well-developed collagen bundles formed the prominent interlobular walls in pigs. In contrast, the wall in the goat liver comprised a thin layer of the bundles. In the chicken liver, there were no notable collagen septa between lobules. The intralobular collagen construction was quite different between the animals, indicating a fragile collagen fibril networks in pigs, a robust framework in goats and dense fabric-like septa in chickens. These results indicate that the distinct collagen frameworks may contribute to the histological strength of the livers in each of the animal species.

Original languageEnglish
Pages (from-to)451-459
Number of pages9
JournalAnimal Science Journal
Volume80
Issue number4
DOIs
Publication statusPublished - Aug 1 2009

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Goats
collagen
Chickens
Swine
Collagen
goats
electrons
Electrons
chickens
liver
swine
Collagen Type III
Liver
Collagen Type I
Connective Tissue
Antibodies
connective tissues
antibodies
Hepatocytes
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Immunohistochemical and scanning electron microscopic comparison of the collagen network constructions between pig, goat and chicken livers. / Nishimura, Shotaro; Sagara, Ayano; Oshima, Ichiro; Ono, Yoshitaka; Iwamoto, Hisao; Okano, Kaoru; Miyachi, Hideyuki; Tabata, Shoji.

In: Animal Science Journal, Vol. 80, No. 4, 01.08.2009, p. 451-459.

Research output: Contribution to journalArticle

Nishimura, Shotaro ; Sagara, Ayano ; Oshima, Ichiro ; Ono, Yoshitaka ; Iwamoto, Hisao ; Okano, Kaoru ; Miyachi, Hideyuki ; Tabata, Shoji. / Immunohistochemical and scanning electron microscopic comparison of the collagen network constructions between pig, goat and chicken livers. In: Animal Science Journal. 2009 ; Vol. 80, No. 4. pp. 451-459.
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