Immunohistochemical localization of cathepsins B, D and L in the rat osteoclast

T. Goto, T. Tsukuba, T. Kiyoshima, Y. Nishimura, K. Kato, K. Yamamoto, T. Tanaka

Research output: Contribution to journalArticle

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Abstract

Immunohistochemical localization of cathepsins B, D and L in the osteoclasts of rat alveolar and femoral bones was investigated by using the avidin-biotin-peroxidase complex method for semithin, 1-μm-thick cryosections. Extracellular immunoreactivity for cathepsins B and L was clearly demonstrated along the bone resorption lacunae; the intensity of the extracellular immunoreactivity of cathepsin L was stronger than that of cathepsin B. However, the intracellular immunoreactivity of both cathepsins was weak compared with that of cathepsin D. The intracellular immunoreactivity of cathespin D in the osteoclasts was clearly observed in the granules and/or vacuoles, but extracellular cathepsin D immunoreactivity was either negligible or not detected along the resorption lacunae. In the adjacent sections stained with anti-cathepsin L or D, extensive extracellular deposition of cathepsin L was found along the bone resorption lacunae, with or without osteoclasts, although the intracellular reactivity of cathepsin L was weak. This is the first morphological study in which cathepsins B and L have been demonstrated to be produced in the osteoclasts and extensively secreted into resorption lacunae, and in which cathepsin D was found to be present in the cells but scantily secreted into the lacunae. These findings suggest that cathepsins B and L directly and effectively participate in the degradation of the bone matrix.

Original languageEnglish
Pages (from-to)411-414
Number of pages4
JournalHistochemistry
Volume99
Issue number5
DOIs
Publication statusPublished - May 1 1993

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cathepsin L
Cathepsin L
cathepsin B
Cathepsin B
Cathepsin D
osteoclasts
Osteoclasts
cathepsin D
rats
bone resorption
resorption
Bone Resorption
bones
cathepsins
avidin
Cathepsins
Bone Matrix
biotin
thighs
Avidin

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Agricultural and Biological Sciences(all)

Cite this

Goto, T., Tsukuba, T., Kiyoshima, T., Nishimura, Y., Kato, K., Yamamoto, K., & Tanaka, T. (1993). Immunohistochemical localization of cathepsins B, D and L in the rat osteoclast. Histochemistry, 99(5), 411-414. https://doi.org/10.1007/BF00717054

Immunohistochemical localization of cathepsins B, D and L in the rat osteoclast. / Goto, T.; Tsukuba, T.; Kiyoshima, T.; Nishimura, Y.; Kato, K.; Yamamoto, K.; Tanaka, T.

In: Histochemistry, Vol. 99, No. 5, 01.05.1993, p. 411-414.

Research output: Contribution to journalArticle

Goto, T, Tsukuba, T, Kiyoshima, T, Nishimura, Y, Kato, K, Yamamoto, K & Tanaka, T 1993, 'Immunohistochemical localization of cathepsins B, D and L in the rat osteoclast', Histochemistry, vol. 99, no. 5, pp. 411-414. https://doi.org/10.1007/BF00717054
Goto, T. ; Tsukuba, T. ; Kiyoshima, T. ; Nishimura, Y. ; Kato, K. ; Yamamoto, K. ; Tanaka, T. / Immunohistochemical localization of cathepsins B, D and L in the rat osteoclast. In: Histochemistry. 1993 ; Vol. 99, No. 5. pp. 411-414.
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