Immunohistochemical localization of osteopontin in human pulp stones

Masami Ninomiya, Mika Ohishi, Jun Ichi Kido, Yasuyoshi Ohsaki, Toshihiko Nagata

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The organic matrix component of human pulp stones was investigated by immunohistochemistry. Two pulp stones were extracted from the upper molar teeth of two patients suffering from irreversible pulpitis. Both were formed in the center of the pulp cavity and located apart from the dentin walls. After demineralization, serial sections of the stones were prepared and subjected to immunohistochemical procedures using specific antibodies to type I collagen and noncollagenous proteins (osteopontin, osteonectin, and osteocalcin), which are reported to be involved in calcified matrix formation. Type I collagen was localized evenly in the stones, indicating that it is a major matrix component of pulp stones. Strong immunostaining of osteopontin appeared in the peripheral area of the stones, whereas osteonectin and osteocalcin were not detected. We previously reported that dental pulp cells produced osteopontin in vitro. Osteopontin has been commonly found in other pathological calcification, such as urinary stones, atherosclerotic plaques, and dental calculus. Taken together, the present findings suggest that osteopontin produced by dental pulp cells is possibly associated with calcification of the pulp stone matrix.

Original languageEnglish
Pages (from-to)269-272
Number of pages4
JournalJournal of Endodontics
Volume27
Issue number4
DOIs
Publication statusPublished - Jan 1 2001

Fingerprint

Dental Pulp Calcification
Osteopontin
Osteonectin
Dental Pulp
Osteocalcin
Collagen Type I
Dental Calculus
Pulpitis
Urinary Calculi
Atherosclerotic Plaques
Dentin
Tooth
Immunohistochemistry
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Ninomiya, M., Ohishi, M., Kido, J. I., Ohsaki, Y., & Nagata, T. (2001). Immunohistochemical localization of osteopontin in human pulp stones. Journal of Endodontics, 27(4), 269-272. https://doi.org/10.1097/00004770-200104000-00007

Immunohistochemical localization of osteopontin in human pulp stones. / Ninomiya, Masami; Ohishi, Mika; Kido, Jun Ichi; Ohsaki, Yasuyoshi; Nagata, Toshihiko.

In: Journal of Endodontics, Vol. 27, No. 4, 01.01.2001, p. 269-272.

Research output: Contribution to journalArticle

Ninomiya, M, Ohishi, M, Kido, JI, Ohsaki, Y & Nagata, T 2001, 'Immunohistochemical localization of osteopontin in human pulp stones', Journal of Endodontics, vol. 27, no. 4, pp. 269-272. https://doi.org/10.1097/00004770-200104000-00007
Ninomiya, Masami ; Ohishi, Mika ; Kido, Jun Ichi ; Ohsaki, Yasuyoshi ; Nagata, Toshihiko. / Immunohistochemical localization of osteopontin in human pulp stones. In: Journal of Endodontics. 2001 ; Vol. 27, No. 4. pp. 269-272.
@article{7846dc4975d34ecda2dce34eec8300b4,
title = "Immunohistochemical localization of osteopontin in human pulp stones",
abstract = "The organic matrix component of human pulp stones was investigated by immunohistochemistry. Two pulp stones were extracted from the upper molar teeth of two patients suffering from irreversible pulpitis. Both were formed in the center of the pulp cavity and located apart from the dentin walls. After demineralization, serial sections of the stones were prepared and subjected to immunohistochemical procedures using specific antibodies to type I collagen and noncollagenous proteins (osteopontin, osteonectin, and osteocalcin), which are reported to be involved in calcified matrix formation. Type I collagen was localized evenly in the stones, indicating that it is a major matrix component of pulp stones. Strong immunostaining of osteopontin appeared in the peripheral area of the stones, whereas osteonectin and osteocalcin were not detected. We previously reported that dental pulp cells produced osteopontin in vitro. Osteopontin has been commonly found in other pathological calcification, such as urinary stones, atherosclerotic plaques, and dental calculus. Taken together, the present findings suggest that osteopontin produced by dental pulp cells is possibly associated with calcification of the pulp stone matrix.",
author = "Masami Ninomiya and Mika Ohishi and Kido, {Jun Ichi} and Yasuyoshi Ohsaki and Toshihiko Nagata",
year = "2001",
month = "1",
day = "1",
doi = "10.1097/00004770-200104000-00007",
language = "English",
volume = "27",
pages = "269--272",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Immunohistochemical localization of osteopontin in human pulp stones

AU - Ninomiya, Masami

AU - Ohishi, Mika

AU - Kido, Jun Ichi

AU - Ohsaki, Yasuyoshi

AU - Nagata, Toshihiko

PY - 2001/1/1

Y1 - 2001/1/1

N2 - The organic matrix component of human pulp stones was investigated by immunohistochemistry. Two pulp stones were extracted from the upper molar teeth of two patients suffering from irreversible pulpitis. Both were formed in the center of the pulp cavity and located apart from the dentin walls. After demineralization, serial sections of the stones were prepared and subjected to immunohistochemical procedures using specific antibodies to type I collagen and noncollagenous proteins (osteopontin, osteonectin, and osteocalcin), which are reported to be involved in calcified matrix formation. Type I collagen was localized evenly in the stones, indicating that it is a major matrix component of pulp stones. Strong immunostaining of osteopontin appeared in the peripheral area of the stones, whereas osteonectin and osteocalcin were not detected. We previously reported that dental pulp cells produced osteopontin in vitro. Osteopontin has been commonly found in other pathological calcification, such as urinary stones, atherosclerotic plaques, and dental calculus. Taken together, the present findings suggest that osteopontin produced by dental pulp cells is possibly associated with calcification of the pulp stone matrix.

AB - The organic matrix component of human pulp stones was investigated by immunohistochemistry. Two pulp stones were extracted from the upper molar teeth of two patients suffering from irreversible pulpitis. Both were formed in the center of the pulp cavity and located apart from the dentin walls. After demineralization, serial sections of the stones were prepared and subjected to immunohistochemical procedures using specific antibodies to type I collagen and noncollagenous proteins (osteopontin, osteonectin, and osteocalcin), which are reported to be involved in calcified matrix formation. Type I collagen was localized evenly in the stones, indicating that it is a major matrix component of pulp stones. Strong immunostaining of osteopontin appeared in the peripheral area of the stones, whereas osteonectin and osteocalcin were not detected. We previously reported that dental pulp cells produced osteopontin in vitro. Osteopontin has been commonly found in other pathological calcification, such as urinary stones, atherosclerotic plaques, and dental calculus. Taken together, the present findings suggest that osteopontin produced by dental pulp cells is possibly associated with calcification of the pulp stone matrix.

UR - http://www.scopus.com/inward/record.url?scp=0035317644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035317644&partnerID=8YFLogxK

U2 - 10.1097/00004770-200104000-00007

DO - 10.1097/00004770-200104000-00007

M3 - Article

C2 - 11485265

AN - SCOPUS:0035317644

VL - 27

SP - 269

EP - 272

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 4

ER -