Immunohistochemical study of desmosomes in oral squamous cell carcinoma: Correlation with cytokeratin and E-cadherin staining, and with tumour behaviour

Masanori Shinohara, Akimitsu Hiraki, Tetsuro Ikebe, Seiji Nakamura, Shin Ichi Kurahara, Kanemitsu Shirasuna, David R. Garrod

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Reduction or loss of the intercellular junctions known as desmosomes may contribute to the invasive and metastatic behaviour of various carcinomas. Previous studies have shown that metastasis of oral squamous cell carcinomas of the head and neck correlates with a reduction in immunohistochemical staining for desmoplakin and desmoglein at the invasion front. The primary aim of the present study was to extend these observations to include a third component of desmosomes, the glycoprotein desmocollin. An additional aim was to determine whether the differentiation status of tumours is reflected in their staining for cytokeratins 1, 13, and 19, and, if so, whether these parameters correlate with desmosomal staining and/or metastasis. The study included 54 primary tumours of which 28 showed lymph node metastases. The results of this investigation show that tumours can be divided into three groups according to whether they have lost staining for no, one or more than one desmosomal component. A statistically significant correlation was found between the number of desmosomal components lost and metastasis. Tumours could also be divided into five groups according to their staining for different combinations of cytokeratins. Furthermore, differentiation status as indicated both histologically and by cytokeratin staining correlated with reduced desmosomal staining and metastasis. Tumours were also examined for intensity of staining for the adhesion molecule E-cadherin. Reduction in E- cadherin staining was correlated with mode of invasion and with reduction in desmosomal staining, but not with poor differentiation as indicated by cytokeratin staining. The results of this extensive study reinforce the view that adhesive junctions and adhesion molecules contribute to the suppression of tumour invasion and metastasis.

Original languageEnglish
Pages (from-to)369-381
Number of pages13
JournalJournal of Pathology
Volume184
Issue number4
DOIs
Publication statusPublished - Apr 1 1998

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Desmosomes
Cadherins
Keratins
Squamous Cell Carcinoma
Staining and Labeling
Neoplasms
Neoplasm Metastasis
Junctional Adhesion Molecules
Desmocollins
Keratin-13
Desmogleins
Desmoplakins
Keratin-19
Intercellular Junctions
Adhesives
Glycoproteins
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Immunohistochemical study of desmosomes in oral squamous cell carcinoma : Correlation with cytokeratin and E-cadherin staining, and with tumour behaviour. / Shinohara, Masanori; Hiraki, Akimitsu; Ikebe, Tetsuro; Nakamura, Seiji; Kurahara, Shin Ichi; Shirasuna, Kanemitsu; Garrod, David R.

In: Journal of Pathology, Vol. 184, No. 4, 01.04.1998, p. 369-381.

Research output: Contribution to journalArticle

Shinohara, Masanori ; Hiraki, Akimitsu ; Ikebe, Tetsuro ; Nakamura, Seiji ; Kurahara, Shin Ichi ; Shirasuna, Kanemitsu ; Garrod, David R. / Immunohistochemical study of desmosomes in oral squamous cell carcinoma : Correlation with cytokeratin and E-cadherin staining, and with tumour behaviour. In: Journal of Pathology. 1998 ; Vol. 184, No. 4. pp. 369-381.
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