Immunohistochemical study on tissue transglutaminase and copper-zinc superoxide dismutase in human myocardium: Its relevance to apoptosis detected by the nick end labelling method

Kenji Kashima, Shigeo Yokoyama, Tsutomu Daa, Iwao Nakayama, Toru Iwaki

Research output: Contribution to journalArticle

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Abstract

The influence of free radicals on apoptosis was studied in the human heart; 45 autopsy cases were examined by the nick end labelling method (NELM) that detects DNA fragmentation. Immunostaining for copper-zinc superoxide dismutase (CuZn-SOD) and tissue transglutaminase (tTG) induced frequently during apoptosis were also studied. Positive immunoreaction for tTG was detected in mucinous degeneration of myocardial cells; these same cells were also positive for CuZn-SOD but negative for NELM. Myocardial cells showing basophilic alterations after haematoxylin and eosin staining were also positive for CuZn-SOD but negative for the other markers examined. Positive nuclear reaction by NELM was only observed in myocardial cells showing contraction band necrosis or irregularly shaped nuclei surrounding recent or long-standing infarcted foci. In these the other two markers were negative. Since mucinous degeneration lacks the distinguishing morphological features of apoptosis, immunoreactive tTG in this lesion may not imply that the cells are undergoing apoptosis. tTG can be induced in non-apoptotic conditions and may not be involved in apoptosis induced by infarction. Histological disassociation between CuZn-SOD expression and apoptosis suggests the possibility of a cytoprotective role played by endogenous CuZn-SOD against free radical generation in the human heart.

Original languageEnglish
Pages (from-to)333-338
Number of pages6
JournalVirchows Archiv
Volume430
Issue number4
DOIs
Publication statusPublished - May 6 1997

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Superoxide Dismutase
Zinc
Copper
Myocardium
Apoptosis
Free Radicals
DNA Fragmentation
Hematoxylin
Eosine Yellowish-(YS)
Infarction
transglutaminase 2
Autopsy
Necrosis
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Immunohistochemical study on tissue transglutaminase and copper-zinc superoxide dismutase in human myocardium : Its relevance to apoptosis detected by the nick end labelling method. / Kashima, Kenji; Yokoyama, Shigeo; Daa, Tsutomu; Nakayama, Iwao; Iwaki, Toru.

In: Virchows Archiv, Vol. 430, No. 4, 06.05.1997, p. 333-338.

Research output: Contribution to journalArticle

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