Immunohistochemistry improves histopathologic diagnosis in primary aldosteronism

Cristia Volpe, Anders Höög, Tadashi Ogishima, Kuniaki Mukai, Ming Lu, Marja Thorén, Bertil Hamberger

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background In primary aldosteronism (PA) the main source of aldosterone hypersecretion is an aldosteroneproducing adenoma (APA) or a bilateral hyperplasia. Histopathology of the adrenal gland from patients with PA has been difficult, as there are no morphological criteria to ascertain which are the cells that produce aldosterone. We therefore applied new specific antibodies to explore which cells in the adrenal contain the enzymes for aldosterone and cortisol production, respectively. Methods Adrenals from 24 patients with PA were studied. After routine preparation, consecutive sections were stained with antibodies for CYP11B1 (cortisol) and CYP11B2 (aldosterone) enzymes. Results APA had a strong immunoreactivity for CYB11B2. In adrenals from seven patients, we found no APA, but several nodules with strong CYB11B2 immunoreactivity, indicating aldosterone-producing nodular hyperplasia. Conclusions Immunohistochemistry of adrenal steroidogenic enzymes provides novel diagnostic information. This may become an important part of routine histopathology, and contribute to improved clinical management in PA.

Original languageEnglish
Pages (from-to)351-354
Number of pages4
JournalJournal of Clinical Pathology
Volume66
Issue number4
DOIs
Publication statusPublished - Apr 1 2013

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Hyperaldosteronism
Aldosterone
Immunohistochemistry
Adenoma
Hyperplasia
Hydrocortisone
Enzymes
Steroid 11-beta-Hydroxylase
Cytochrome P-450 CYP11B2
Antibodies
Adrenal Glands

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Immunohistochemistry improves histopathologic diagnosis in primary aldosteronism. / Volpe, Cristia; Höög, Anders; Ogishima, Tadashi; Mukai, Kuniaki; Lu, Ming; Thorén, Marja; Hamberger, Bertil.

In: Journal of Clinical Pathology, Vol. 66, No. 4, 01.04.2013, p. 351-354.

Research output: Contribution to journalArticle

Volpe, Cristia ; Höög, Anders ; Ogishima, Tadashi ; Mukai, Kuniaki ; Lu, Ming ; Thorén, Marja ; Hamberger, Bertil. / Immunohistochemistry improves histopathologic diagnosis in primary aldosteronism. In: Journal of Clinical Pathology. 2013 ; Vol. 66, No. 4. pp. 351-354.
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AU - Thorén, Marja

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AB - Background In primary aldosteronism (PA) the main source of aldosterone hypersecretion is an aldosteroneproducing adenoma (APA) or a bilateral hyperplasia. Histopathology of the adrenal gland from patients with PA has been difficult, as there are no morphological criteria to ascertain which are the cells that produce aldosterone. We therefore applied new specific antibodies to explore which cells in the adrenal contain the enzymes for aldosterone and cortisol production, respectively. Methods Adrenals from 24 patients with PA were studied. After routine preparation, consecutive sections were stained with antibodies for CYP11B1 (cortisol) and CYP11B2 (aldosterone) enzymes. Results APA had a strong immunoreactivity for CYB11B2. In adrenals from seven patients, we found no APA, but several nodules with strong CYB11B2 immunoreactivity, indicating aldosterone-producing nodular hyperplasia. Conclusions Immunohistochemistry of adrenal steroidogenic enzymes provides novel diagnostic information. This may become an important part of routine histopathology, and contribute to improved clinical management in PA.

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