Immunolocalization of spetex-1 at the connecting piece in spermatozoa of the musk shrew (suncus murinus)

Takane Kaneko, Shizuka Iwamoto, Emi Murayama, Hitoshi Kurio, Tetsuichiro Inai, Senichi Oda, Hiroshi Iida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Spetex-1, which has been isolated by differential display and rat cDNA library screening as a haploid spermatid-specific gene, encodes a protein with two coiled-coil motifs that locates at both the segmented column in the connecting piece and outer dense fibers-affiliated satellite fibrils in rat sperm flagella. Orthologs of Spetex-1 are identified in many animal species, including human, chimpanzee, macaque, cow, dog, African clawed frog, green spotted puffer, and zebrafish. In this study, we used RT-PCR in combination with 5′and 3′ RACE (Rapid Amplification of cDNA End) technique to isolate Spetex-1 ortholog of the musk shrew (Suneus murinus), which yielded a full-length Suncus Spetex-1 gene containing an open reading frame of 1,908 base pairs encoding a protein of 636 amino acids with the predicted molecular mass of 72,348 Da. Suncus Spetex-1 has two coiled-coil motifs at 118-184 and 242-276 amino acid residues, which is a characteristic shared by mammalian Spetex-1 proteins. To examine the subcellular localization of Spetex-1 in Suncus spermatozoa, we produced the anti-Suncus Spetex-1 antibody and carried out immunocytochemistry. In spite of that the primary structure of Suncus Spetex-1 is basically similar to that of rat and mouse Spetex-1, confocal laser scanning microscopy and immunoelectron microscopy revealed that Spetex-1 was restricted to the segmented column and capitulum in the connecting piece of Suncus spermatozoa and was not detected in other parts of flagella, suggesting a diversity of Spetex-1 localization in mammalian spermatozoa.

Original languageEnglish
Pages (from-to)444-452
Number of pages9
JournalZoological Science
Volume28
Issue number6
DOIs
Publication statusPublished - Jun 2011

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Suncus
Suncus murinus
shrews
spermatozoa
flagellum
rats
Tetraodontidae
amino acids
rapid amplification of cDNA ends
proteins
spermatids
Xenopus laevis
confocal laser scanning microscopy
immunocytochemistry
Danio rerio
Pan troglodytes
Macaca
cDNA libraries
haploidy
open reading frames

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Immunolocalization of spetex-1 at the connecting piece in spermatozoa of the musk shrew (suncus murinus). / Kaneko, Takane; Iwamoto, Shizuka; Murayama, Emi; Kurio, Hitoshi; Inai, Tetsuichiro; Oda, Senichi; Iida, Hiroshi.

In: Zoological Science, Vol. 28, No. 6, 06.2011, p. 444-452.

Research output: Contribution to journalArticle

Kaneko, Takane ; Iwamoto, Shizuka ; Murayama, Emi ; Kurio, Hitoshi ; Inai, Tetsuichiro ; Oda, Senichi ; Iida, Hiroshi. / Immunolocalization of spetex-1 at the connecting piece in spermatozoa of the musk shrew (suncus murinus). In: Zoological Science. 2011 ; Vol. 28, No. 6. pp. 444-452.
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