Sexual aggregation involved in conjugative transfer of the Enterococcus faecalis plasmids pPDl and pADl is enhanced by sex pheromones cPDl and cADl, respectively, which are excreted from recipient cells. PD78 (78kDa) and AD74 (74kDa) were detectable on the surface of donors harboring pPDl and pAD1, respectively, at the time of cell aggregation. The proteins PD78 and AD74 were purified and used to raise anti-PD78 and anti-AD74 antibodies. The antibodies blocked the sexual aggregation and the plasmid transfer. Anti-AD74 antibody reacted to both 153 kDa proteins extracted from cPDl and cADl-induced donor cells after lysozyme digestion of cell wall. Pheromone-induced synthesis of PD78 and AD74, when both plasmids were present in the same cell, was independent.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry