Immunopotentiating activity of nigerooligosaccharides for the t helper 1-like immune response in mice

Shinji Murosaki, Koutarou Muroyama, Yoshihiro Yamamoto, Hiroaki Kusaka, Tiei Liu, Yasunobu Yoshikai

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    48 Citations (Scopus)

    Abstract

    The immunopotentiating activity of nigerooligosaccharides (NOS), a mixture of nigerose, nigerosyl glucose and nigerosyl maltose, was studied in vitro and in vivo in mice. Mitogen-induced proliferation of splenocytes from normal mice was augmented in a dose-dependent manner by nigerose of NOS. NOS enhanced interleukin 12 (IL-12) and interferon-γ (IFN-γ) production by normal splenocytes in the presence of the potent IL-12 inducer, heat-killed Lactobacillus plantarum L-137, in vitro. Consistent with the in vitro finding, L. plantarum L-137-induced IL-12 production and IL-2-induced IFN-γ production were augmented in mice fed with a 14.6% NOS diet for 2 weeks compared with mice fed with a control diet. Notably, mice fed with the NOS diet showed significantly longer survival time than the control mice after the induction of an endogenous infection by administering 5-fluorouracil in a lethal dose. Taken together, these results suggest that NOS may exert immunopotentiating activity through the activation of an IL-12-dependent T helper 1-like immune response.

    Original languageEnglish
    Pages (from-to)373-378
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume63
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 1999

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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