Abstract
Isolated hypoganglionosis (IHG) has been proposed as a distinct entity with two subtypes: congenital IHG (CIHG) and acquired IHG (AIHG). However, due to the rarity of the disease and the lack of defining histological criteria, the concept of IHG is not widely accepted. We studied paraffin-embedded intestinal specimens from 79 patients diagnosed with Hirschsprung’s disease (HD) (n = 49), CIHG (n = 25), and AIHG (n = 5) collected between January 1996 and December 2015. Histopathological diagnosis of HD, CIHG, and AIHG was confirmed by hematoxylin and eosin staining and immunohistochemical staining using Hu C/D and CD56. We evaluated (immuno)histopathological findings, counted the number of ganglion cells, and measured the size of Auerbach’s plexus. Hu C/D labeled neuronal cell bodies, whereas CD56 was detected in all neuronal components. In HD, all ganglion cells in Auerbach’s plexus in the normoganglionic segment (NGS) were immunoreactive for Hu C/D, whereas in the aganglionic segment (AGS), these were replaced by CD56-positive extrinsic nerve fibers and bundles. The number of ganglion cells in AIHG and CIHG was significantly lower than in the NGS of HD (p < 0.05). Auerbach’s plexus was significantly smaller in CIHG (p < 0.05) but in AIHG equivalent to the NGS in HD. In summary, immunostaining for Hu C/D and CD56 is useful for definitive histopathological diagnosis of IHG.
| Original language | English |
|---|---|
| Pages (from-to) | 679-685 |
| Number of pages | 7 |
| Journal | Virchows Archiv |
| Volume | 470 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 1 2017 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Molecular Biology
- Cell Biology
Cite this
Immunostaining for Hu C/D and CD56 is useful for a definitive histopathological diagnosis of congenital and acquired isolated hypoganglionosis. / Yoshimaru, Koichiro; Taguchi, Tomoaki; Obata, Satoshi; Takemoto, Junkichi; Takahashi, Yoshiaki; Iwanaka, Tsuyoshi; Yanagi, Yusuke; Kuda, Masaaki; Miyoshi, Kina; Matsuura, Toshiharu; Kinoshita, Yoshiaki; Yoshioka, Takako; Nakazawa, Atsuko; Oda, Yoshinao.
In: Virchows Archiv, Vol. 470, No. 6, 01.06.2017, p. 679-685.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunostaining for Hu C/D and CD56 is useful for a definitive histopathological diagnosis of congenital and acquired isolated hypoganglionosis
AU - Yoshimaru, Koichiro
AU - Taguchi, Tomoaki
AU - Obata, Satoshi
AU - Takemoto, Junkichi
AU - Takahashi, Yoshiaki
AU - Iwanaka, Tsuyoshi
AU - Yanagi, Yusuke
AU - Kuda, Masaaki
AU - Miyoshi, Kina
AU - Matsuura, Toshiharu
AU - Kinoshita, Yoshiaki
AU - Yoshioka, Takako
AU - Nakazawa, Atsuko
AU - Oda, Yoshinao
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Isolated hypoganglionosis (IHG) has been proposed as a distinct entity with two subtypes: congenital IHG (CIHG) and acquired IHG (AIHG). However, due to the rarity of the disease and the lack of defining histological criteria, the concept of IHG is not widely accepted. We studied paraffin-embedded intestinal specimens from 79 patients diagnosed with Hirschsprung’s disease (HD) (n = 49), CIHG (n = 25), and AIHG (n = 5) collected between January 1996 and December 2015. Histopathological diagnosis of HD, CIHG, and AIHG was confirmed by hematoxylin and eosin staining and immunohistochemical staining using Hu C/D and CD56. We evaluated (immuno)histopathological findings, counted the number of ganglion cells, and measured the size of Auerbach’s plexus. Hu C/D labeled neuronal cell bodies, whereas CD56 was detected in all neuronal components. In HD, all ganglion cells in Auerbach’s plexus in the normoganglionic segment (NGS) were immunoreactive for Hu C/D, whereas in the aganglionic segment (AGS), these were replaced by CD56-positive extrinsic nerve fibers and bundles. The number of ganglion cells in AIHG and CIHG was significantly lower than in the NGS of HD (p < 0.05). Auerbach’s plexus was significantly smaller in CIHG (p < 0.05) but in AIHG equivalent to the NGS in HD. In summary, immunostaining for Hu C/D and CD56 is useful for definitive histopathological diagnosis of IHG.
AB - Isolated hypoganglionosis (IHG) has been proposed as a distinct entity with two subtypes: congenital IHG (CIHG) and acquired IHG (AIHG). However, due to the rarity of the disease and the lack of defining histological criteria, the concept of IHG is not widely accepted. We studied paraffin-embedded intestinal specimens from 79 patients diagnosed with Hirschsprung’s disease (HD) (n = 49), CIHG (n = 25), and AIHG (n = 5) collected between January 1996 and December 2015. Histopathological diagnosis of HD, CIHG, and AIHG was confirmed by hematoxylin and eosin staining and immunohistochemical staining using Hu C/D and CD56. We evaluated (immuno)histopathological findings, counted the number of ganglion cells, and measured the size of Auerbach’s plexus. Hu C/D labeled neuronal cell bodies, whereas CD56 was detected in all neuronal components. In HD, all ganglion cells in Auerbach’s plexus in the normoganglionic segment (NGS) were immunoreactive for Hu C/D, whereas in the aganglionic segment (AGS), these were replaced by CD56-positive extrinsic nerve fibers and bundles. The number of ganglion cells in AIHG and CIHG was significantly lower than in the NGS of HD (p < 0.05). Auerbach’s plexus was significantly smaller in CIHG (p < 0.05) but in AIHG equivalent to the NGS in HD. In summary, immunostaining for Hu C/D and CD56 is useful for definitive histopathological diagnosis of IHG.
UR - http://www.scopus.com/inward/record.url?scp=85018499230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018499230&partnerID=8YFLogxK
U2 - 10.1007/s00428-017-2128-9
DO - 10.1007/s00428-017-2128-9
M3 - Article
C2 - 28424865
AN - SCOPUS:85018499230
VL - 470
SP - 679
EP - 685
JO - Virchows Archiv
JF - Virchows Archiv
SN - 0945-6317
IS - 6
ER -