Immunosuppressive effect of nucleoside-nucleotide-free diet in rat allogeneic small intestinal transplantation

K. Ogita, S. Suita, T. Taguchi, T. Uesugi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background We evaluated the effects of nucleosides (NS) and nucleotides (NT) on the rejection of rat allogeneic small intestinal transplants. Methods A 2-cm segment of jejunum from fetal Fischer rats (RT-1lvl) was transplanted at day 19 of gestation into the abdominal wall of 7-week-old Lewis rats (RT-1l) by a nonvascular technique. Two weeks before transplantation, recipient rats were separated into an NS-NT-free group and an NS-NT-supplemented group. At 2 days after transplantation, histologic study of the grafts was performed with hematoxylin-eosin staining and interleukin-2 (IL-2) production estimated in recipient blood using an ELISA method. The morphologic findings were graded in a blind fashion on a scale of 0 to 4, with 0 indicating an intact intestinal structure. Results Mean plasma IL-2 levels of the NS-NT-free group were significantly lower than those of the NS-NT-supplemented group. The mean rejection score of the NS-NT-free group was also significantly lower than that of the NS-NT-supplemented group. Conclusions Administration of an NS-NT-free diet reduces acute rejection in rat small intestinal transplantations.

Original languageEnglish
Pages (from-to)329-330
Number of pages2
JournalTransplantation Proceedings
Volume36
Issue number2
DOIs
Publication statusPublished - Mar 2004

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

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