Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics

Minoru Takeshita, Yuka Matsuo, Masashi Suzuki, Naruto Furuya, Kenichi Tsuchiya, Yoichi Takanami

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

D RNA 3Yα (D3Yα), a defective (D) RNA 3 derived from the Y strain of cucumber mosaic virus (CMV-Y), was further characterized in combination with different helper viruses in the genus Cucumovirus. Interestingly, Nicotiana benthamiana plants inoculated with CMV-D8 and D3Yα developed systemic symptoms which were different from those induced by CMV-D8. To elucidate the potential effects of D RNA 3 on virus infection on the basis of the original combination of CMV-Y and D3Yα, a point mutation was made in the coat protein gene, which determined symptoms, of either CMV-Y RNA 3 (Y3) or D3Yα. Symptoms induced on N. benthamiana and N. tabacum plants, and semi-quantitative RT-PCR revealed that the ratio of RNA 3 to D RNA 3 was associated with the differences of symptoms in the leaf tissues. Furthermore, analysis of in situ hybridization suggested that there were spatial effects between coat proteins of Y3 and D3Yα in the infected leaves.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalVirology
Volume389
Issue number1-2
DOIs
Publication statusPublished - Jun 2 2009

Fingerprint

Cucumovirus
Helper Viruses
Virus Diseases
RNA
Capsid Proteins
DNA-like RNA
Point Mutation
Tobacco
In Situ Hybridization
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics. / Takeshita, Minoru; Matsuo, Yuka; Suzuki, Masashi; Furuya, Naruto; Tsuchiya, Kenichi; Takanami, Yoichi.

In: Virology, Vol. 389, No. 1-2, 02.06.2009, p. 59-65.

Research output: Contribution to journalArticle

Takeshita, M, Matsuo, Y, Suzuki, M, Furuya, N, Tsuchiya, K & Takanami, Y 2009, 'Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics', Virology, vol. 389, no. 1-2, pp. 59-65. https://doi.org/10.1016/j.virol.2009.04.010
Takeshita, Minoru ; Matsuo, Yuka ; Suzuki, Masashi ; Furuya, Naruto ; Tsuchiya, Kenichi ; Takanami, Yoichi. / Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics. In: Virology. 2009 ; Vol. 389, No. 1-2. pp. 59-65.
@article{fd8c2a661ed34c3594c35ed5a4cb4ab0,
title = "Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics",
abstract = "D RNA 3Yα (D3Yα), a defective (D) RNA 3 derived from the Y strain of cucumber mosaic virus (CMV-Y), was further characterized in combination with different helper viruses in the genus Cucumovirus. Interestingly, Nicotiana benthamiana plants inoculated with CMV-D8 and D3Yα developed systemic symptoms which were different from those induced by CMV-D8. To elucidate the potential effects of D RNA 3 on virus infection on the basis of the original combination of CMV-Y and D3Yα, a point mutation was made in the coat protein gene, which determined symptoms, of either CMV-Y RNA 3 (Y3) or D3Yα. Symptoms induced on N. benthamiana and N. tabacum plants, and semi-quantitative RT-PCR revealed that the ratio of RNA 3 to D RNA 3 was associated with the differences of symptoms in the leaf tissues. Furthermore, analysis of in situ hybridization suggested that there were spatial effects between coat proteins of Y3 and D3Yα in the infected leaves.",
author = "Minoru Takeshita and Yuka Matsuo and Masashi Suzuki and Naruto Furuya and Kenichi Tsuchiya and Yoichi Takanami",
year = "2009",
month = "6",
day = "2",
doi = "10.1016/j.virol.2009.04.010",
language = "English",
volume = "389",
pages = "59--65",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1-2",

}

TY - JOUR

T1 - Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics

AU - Takeshita, Minoru

AU - Matsuo, Yuka

AU - Suzuki, Masashi

AU - Furuya, Naruto

AU - Tsuchiya, Kenichi

AU - Takanami, Yoichi

PY - 2009/6/2

Y1 - 2009/6/2

N2 - D RNA 3Yα (D3Yα), a defective (D) RNA 3 derived from the Y strain of cucumber mosaic virus (CMV-Y), was further characterized in combination with different helper viruses in the genus Cucumovirus. Interestingly, Nicotiana benthamiana plants inoculated with CMV-D8 and D3Yα developed systemic symptoms which were different from those induced by CMV-D8. To elucidate the potential effects of D RNA 3 on virus infection on the basis of the original combination of CMV-Y and D3Yα, a point mutation was made in the coat protein gene, which determined symptoms, of either CMV-Y RNA 3 (Y3) or D3Yα. Symptoms induced on N. benthamiana and N. tabacum plants, and semi-quantitative RT-PCR revealed that the ratio of RNA 3 to D RNA 3 was associated with the differences of symptoms in the leaf tissues. Furthermore, analysis of in situ hybridization suggested that there were spatial effects between coat proteins of Y3 and D3Yα in the infected leaves.

AB - D RNA 3Yα (D3Yα), a defective (D) RNA 3 derived from the Y strain of cucumber mosaic virus (CMV-Y), was further characterized in combination with different helper viruses in the genus Cucumovirus. Interestingly, Nicotiana benthamiana plants inoculated with CMV-D8 and D3Yα developed systemic symptoms which were different from those induced by CMV-D8. To elucidate the potential effects of D RNA 3 on virus infection on the basis of the original combination of CMV-Y and D3Yα, a point mutation was made in the coat protein gene, which determined symptoms, of either CMV-Y RNA 3 (Y3) or D3Yα. Symptoms induced on N. benthamiana and N. tabacum plants, and semi-quantitative RT-PCR revealed that the ratio of RNA 3 to D RNA 3 was associated with the differences of symptoms in the leaf tissues. Furthermore, analysis of in situ hybridization suggested that there were spatial effects between coat proteins of Y3 and D3Yα in the infected leaves.

UR - http://www.scopus.com/inward/record.url?scp=67349282267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349282267&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2009.04.010

DO - 10.1016/j.virol.2009.04.010

M3 - Article

C2 - 19427011

AN - SCOPUS:67349282267

VL - 389

SP - 59

EP - 65

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1-2

ER -