Impact of melatonin and molecular clockwork components on the expression of thyrotropin β-chain (Tshb) and the Tsh receptor in the mouse pars tuberalis

Claudia Unfried, Nariman Ansari, Shinobu Yasuo, Horst Werner Korf, Charlotte Von Gall

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Photoperiodic regulation of reproduction in birds and mammals involves thyrotropin β-chain (TSHb), which is secreted from the pars tuberalis (PT) and controls the expression of deiodinase type 2 and 3 in the ependymal cell layer of the infundibular recess (EC) via TSH receptors (TSHRs). To analyze the impact of melatonin and the molecular clockwork on the expression of Tshb and Tshr, we investigated melatonin-proficient C3H wild-type (WT), melatonin receptor 1-deficient (MT1-/-) or clockprotein PERIOD1-deficient (mPER1-/-) mice. Expression of Tshb and TSHb immunoreactivity in PT were low during day and high during the night in WT, high during the day and low during the night in mPER1-deficient, and equally high during the day and night in MT1-deficient mice. Melatonin injections into WT acutely suppressed Tshb expression. Transcription assays showed that the 5′ upstream region of the Tshb gene could be controlled by clockproteins. Tshr levels in PT were low during the day and high during the night in WT and mPER1-deficient mice and equally low in MT1-deficient mice. Tshr expression in the EC did not show a day/night variation. Melatonin injections into WT acutely induced Tshr expression in PT but not in EC. TSH stimulation of hypothalamic slice cultures of WT induced phosphorylated cAMP response element-binding protein in PT and EC and deiodinase type 2 in the EC. Our data suggest that Tshb expression in PT is controlled by melatonin and the molecular clockwork and that melatonin activates Tshr expression in PT but not in EC. They also confirm the functional importance of TSHR in the PT and EC.

Original languageEnglish
Pages (from-to)4653-4662
Number of pages10
JournalEndocrinology
Volume150
Issue number10
DOIs
Publication statusPublished - Oct 1 2009

Fingerprint

Thyrotropin
Melatonin
Thyrotropin Receptors
Iodide Peroxidase
Melatonin Receptors
Cyclic AMP Response Element-Binding Protein
Injections
Birds
Reproduction
Mammals
Genes

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Impact of melatonin and molecular clockwork components on the expression of thyrotropin β-chain (Tshb) and the Tsh receptor in the mouse pars tuberalis. / Unfried, Claudia; Ansari, Nariman; Yasuo, Shinobu; Korf, Horst Werner; Von Gall, Charlotte.

In: Endocrinology, Vol. 150, No. 10, 01.10.2009, p. 4653-4662.

Research output: Contribution to journalArticle

Unfried, Claudia ; Ansari, Nariman ; Yasuo, Shinobu ; Korf, Horst Werner ; Von Gall, Charlotte. / Impact of melatonin and molecular clockwork components on the expression of thyrotropin β-chain (Tshb) and the Tsh receptor in the mouse pars tuberalis. In: Endocrinology. 2009 ; Vol. 150, No. 10. pp. 4653-4662.
@article{8b2800fad62e410d9adb1f100d896dea,
title = "Impact of melatonin and molecular clockwork components on the expression of thyrotropin β-chain (Tshb) and the Tsh receptor in the mouse pars tuberalis",
abstract = "Photoperiodic regulation of reproduction in birds and mammals involves thyrotropin β-chain (TSHb), which is secreted from the pars tuberalis (PT) and controls the expression of deiodinase type 2 and 3 in the ependymal cell layer of the infundibular recess (EC) via TSH receptors (TSHRs). To analyze the impact of melatonin and the molecular clockwork on the expression of Tshb and Tshr, we investigated melatonin-proficient C3H wild-type (WT), melatonin receptor 1-deficient (MT1-/-) or clockprotein PERIOD1-deficient (mPER1-/-) mice. Expression of Tshb and TSHb immunoreactivity in PT were low during day and high during the night in WT, high during the day and low during the night in mPER1-deficient, and equally high during the day and night in MT1-deficient mice. Melatonin injections into WT acutely suppressed Tshb expression. Transcription assays showed that the 5′ upstream region of the Tshb gene could be controlled by clockproteins. Tshr levels in PT were low during the day and high during the night in WT and mPER1-deficient mice and equally low in MT1-deficient mice. Tshr expression in the EC did not show a day/night variation. Melatonin injections into WT acutely induced Tshr expression in PT but not in EC. TSH stimulation of hypothalamic slice cultures of WT induced phosphorylated cAMP response element-binding protein in PT and EC and deiodinase type 2 in the EC. Our data suggest that Tshb expression in PT is controlled by melatonin and the molecular clockwork and that melatonin activates Tshr expression in PT but not in EC. They also confirm the functional importance of TSHR in the PT and EC.",
author = "Claudia Unfried and Nariman Ansari and Shinobu Yasuo and Korf, {Horst Werner} and {Von Gall}, Charlotte",
year = "2009",
month = "10",
day = "1",
doi = "10.1210/en.2009-0609",
language = "English",
volume = "150",
pages = "4653--4662",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "10",

}

TY - JOUR

T1 - Impact of melatonin and molecular clockwork components on the expression of thyrotropin β-chain (Tshb) and the Tsh receptor in the mouse pars tuberalis

AU - Unfried, Claudia

AU - Ansari, Nariman

AU - Yasuo, Shinobu

AU - Korf, Horst Werner

AU - Von Gall, Charlotte

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Photoperiodic regulation of reproduction in birds and mammals involves thyrotropin β-chain (TSHb), which is secreted from the pars tuberalis (PT) and controls the expression of deiodinase type 2 and 3 in the ependymal cell layer of the infundibular recess (EC) via TSH receptors (TSHRs). To analyze the impact of melatonin and the molecular clockwork on the expression of Tshb and Tshr, we investigated melatonin-proficient C3H wild-type (WT), melatonin receptor 1-deficient (MT1-/-) or clockprotein PERIOD1-deficient (mPER1-/-) mice. Expression of Tshb and TSHb immunoreactivity in PT were low during day and high during the night in WT, high during the day and low during the night in mPER1-deficient, and equally high during the day and night in MT1-deficient mice. Melatonin injections into WT acutely suppressed Tshb expression. Transcription assays showed that the 5′ upstream region of the Tshb gene could be controlled by clockproteins. Tshr levels in PT were low during the day and high during the night in WT and mPER1-deficient mice and equally low in MT1-deficient mice. Tshr expression in the EC did not show a day/night variation. Melatonin injections into WT acutely induced Tshr expression in PT but not in EC. TSH stimulation of hypothalamic slice cultures of WT induced phosphorylated cAMP response element-binding protein in PT and EC and deiodinase type 2 in the EC. Our data suggest that Tshb expression in PT is controlled by melatonin and the molecular clockwork and that melatonin activates Tshr expression in PT but not in EC. They also confirm the functional importance of TSHR in the PT and EC.

AB - Photoperiodic regulation of reproduction in birds and mammals involves thyrotropin β-chain (TSHb), which is secreted from the pars tuberalis (PT) and controls the expression of deiodinase type 2 and 3 in the ependymal cell layer of the infundibular recess (EC) via TSH receptors (TSHRs). To analyze the impact of melatonin and the molecular clockwork on the expression of Tshb and Tshr, we investigated melatonin-proficient C3H wild-type (WT), melatonin receptor 1-deficient (MT1-/-) or clockprotein PERIOD1-deficient (mPER1-/-) mice. Expression of Tshb and TSHb immunoreactivity in PT were low during day and high during the night in WT, high during the day and low during the night in mPER1-deficient, and equally high during the day and night in MT1-deficient mice. Melatonin injections into WT acutely suppressed Tshb expression. Transcription assays showed that the 5′ upstream region of the Tshb gene could be controlled by clockproteins. Tshr levels in PT were low during the day and high during the night in WT and mPER1-deficient mice and equally low in MT1-deficient mice. Tshr expression in the EC did not show a day/night variation. Melatonin injections into WT acutely induced Tshr expression in PT but not in EC. TSH stimulation of hypothalamic slice cultures of WT induced phosphorylated cAMP response element-binding protein in PT and EC and deiodinase type 2 in the EC. Our data suggest that Tshb expression in PT is controlled by melatonin and the molecular clockwork and that melatonin activates Tshr expression in PT but not in EC. They also confirm the functional importance of TSHR in the PT and EC.

UR - http://www.scopus.com/inward/record.url?scp=70349339384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349339384&partnerID=8YFLogxK

U2 - 10.1210/en.2009-0609

DO - 10.1210/en.2009-0609

M3 - Article

C2 - 19589858

AN - SCOPUS:70349339384

VL - 150

SP - 4653

EP - 4662

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 10

ER -