Impact of monocyte chemoattractant protein-1 deficiency on cerebral aneurysm formation

Tomohiro Aoki, Hiroharu Kataoka, Ryota Ishibashi, Kazuhiko Nozaki, Kensuke Egashira, Nobuo Hashimoto

    Research output: Contribution to journalArticlepeer-review

    143 Citations (Scopus)

    Abstract

    Background and Purpose - Recent studies have suggested that chronic inflammation actively participates in cerebral aneurysm (CA) formation. Macrophages accumulate in CA walls and express proinflammatory genes promoting CA progression, but the molecular mechanisms of monocyte/macrophage recruitment into CA walls remain to be elucidated. Methods - Monocyte chemoattractant protein-1 (MCP-1) expression in experimentally induced CAs was assessed by immunohistochemistry and Western blotting. The role of MCP-1 in CA formation was examined by MCP-1-/- mice and a plasmid DNA encoding a dominant negative mutant of MCP-1 (7ND). MCP-1 expression in human CAs was examined by immunohistochemistry. Results - MCP-1 expression was upregulated in aneurysmal walls at the early stage of CA formation. MCP-1-/- mice exhibited a significant decrease of CA formation and macrophage accumulation with decreased expression of matrix metalloproteinase-2, -9, and inducible nitric oxide synthase. Immunohistochenustry for the DNA binding form of nuclear factor-kappa B showed nuclear factor-kappa B activation in MCP-1-expressing cells. Blockade of MCP-1 activity by 7ND resulted in the inhibition of CA progression in rats. In human CAs, MCP-1 was also expressed in CA walls. Conclusions - These data suggest that MCP-1 plays a crucial role in CA formation as a major chemoattractant for monocyte/macrophage. MCP-1 expression in CA walls is induced through nuclear factor-kappa B activation. MCP-1 may be a novel therapeutic target of medical treatment preventing CA progression.

    Original languageEnglish
    Pages (from-to)942-951
    Number of pages10
    JournalStroke
    Volume40
    Issue number3
    DOIs
    Publication statusPublished - Mar 2009

    All Science Journal Classification (ASJC) codes

    • Clinical Neurology
    • Cardiology and Cardiovascular Medicine
    • Advanced and Specialised Nursing

    Fingerprint Dive into the research topics of 'Impact of monocyte chemoattractant protein-1 deficiency on cerebral aneurysm formation'. Together they form a unique fingerprint.

    Cite this