Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells

Miyako Udono, Kaoru Fujii, Gakuro Harada, Yumi Tsuzuki, Keishi Kadooka, Pingbo Zhang, Hiroshi Fujii, Maho Amano, Shin Ichiro Nishimura, Kosuke Tashiro, Satoru Kuhara, Yoshinori Katakura

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Abstract

Many genes and signaling pathways have been found to be involved in cellular senescence program. In the present study, we have identified 16 senescence-associated genes by differential proteomic analysis of the normal human diploid fibroblast cell line, TIG-1, and focused on ATP6V0A2. The aim of this study is to clarify the role of ATP6V0A2, the causal gene for ARCL2, a syndrome of abnormal glycosylation and impaired Golgi trafficking, in cellular senescence program. Here we showed that ATP6V0A2 is critical for cellular senescence; impaired expression of ATP6V0A2 disperses the Golgi structure and triggers senescence, suggesting that ATP6V0A2 mediates these processes. FITC-lectin staining and glycoblotting revealed significantly different glycosylation structures in presenescent (young) and senescent (old) TIG-1 cells; reducing ATP6V0A2 expression in young TIG-1 cells yielded structures similar to those in old TIG-1 cells. Our results suggest that senescence-associated impaired expression of ATP6V0A2 triggers changes in Golgi structure and glycosylation in old TIG-1 cells, which demonstrates a role of ATP6V0A2 in cellular senescence program.

Original languageEnglish
Article number17342
JournalScientific reports
Volume5
DOIs
Publication statusPublished - Nov 27 2015

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Cell Aging
Glycosylation
Genes
Fluorescein-5-isothiocyanate
Diploidy
Lectins
Proteomics
Fibroblasts
Staining and Labeling
Cell Line

All Science Journal Classification (ASJC) codes

  • General

Cite this

Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells. / Udono, Miyako; Fujii, Kaoru; Harada, Gakuro; Tsuzuki, Yumi; Kadooka, Keishi; Zhang, Pingbo; Fujii, Hiroshi; Amano, Maho; Nishimura, Shin Ichiro; Tashiro, Kosuke; Kuhara, Satoru; Katakura, Yoshinori.

In: Scientific reports, Vol. 5, 17342, 27.11.2015.

Research output: Contribution to journalArticle

Udono, M, Fujii, K, Harada, G, Tsuzuki, Y, Kadooka, K, Zhang, P, Fujii, H, Amano, M, Nishimura, SI, Tashiro, K, Kuhara, S & Katakura, Y 2015, 'Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells' Scientific reports, vol. 5, 17342. https://doi.org/10.1038/srep17342
Udono, Miyako ; Fujii, Kaoru ; Harada, Gakuro ; Tsuzuki, Yumi ; Kadooka, Keishi ; Zhang, Pingbo ; Fujii, Hiroshi ; Amano, Maho ; Nishimura, Shin Ichiro ; Tashiro, Kosuke ; Kuhara, Satoru ; Katakura, Yoshinori. / Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells. In: Scientific reports. 2015 ; Vol. 5.
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