Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice

Kosuke Watari, Tomohiro Shibata, Hiroshi Nabeshima, Ai Shinoda, Yuichi Fukunaga, Akihiko Kawahara, Kazuyuki Karasuyama, Jun-Ichi Fukushi, Yukihide Iwamoto, Michihiko Kuwano, Mayumi Ono

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Abstract

N-myc downstream regulated gene 1 (NDRG1) is a responsible gene for a hereditary motor and sensory neuropathy-Lom (Charcot-Marie-Tooth disease type 4D). This is the first study aiming to assess the contribution of NDRG1 to differentiation of macrophage lineage cells, which has important implications for bone remodeling and inflammatory angiogenesis. Ndrg1 knockout (KO) mice exhibited abnormal curvature of the spine, high trabecular bone mass, and reduced number of osteoclasts. We observed that serum levels of macrophage colony-stimulating factor (M-CSF) and macrophage-related cytokines were markedly decreased in KO mice. Differentiation of bone marrow (BM) cells into osteoclasts, M1/M2-type macrophages and dendritic cells was all impaired. Furthermore, KO mice also showed reduced tumor growth and angiogenesis by cancer cells, accompanied by decreased infiltration of tumor-associated macrophages. The transfer of BM-derived macrophages from KO mice into BM-eradicated wild type (WT) mice induced much less tumor angiogenesis than observed in WT mice. Angiogenesis in corneas in response to inflammatory stimuli was also suppressed with decreased infiltration of macrophages. Taken together, these results indicate that NDRG1 deficiency attenuates the differentiation of macrophage lineage cells, suppressing bone remodeling and inflammatory angiogenesis. This study strongly suggests the crucial role of NDRG1 in differentiation process for macrophages.

Original languageEnglish
Article number19470
JournalScientific reports
Volume6
DOIs
Publication statusPublished - Jan 18 2016

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Bone Remodeling
Macrophages
Knockout Mice
Genes
Osteoclasts
Neoplasms
Hereditary Sensory and Motor Neuropathy
Macrophage Colony-Stimulating Factor
Bone Marrow Cells
Cornea
Dendritic Cells
Spine
Bone Marrow
Cytokines
Growth
Serum

All Science Journal Classification (ASJC) codes

  • General

Cite this

Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice. / Watari, Kosuke; Shibata, Tomohiro; Nabeshima, Hiroshi; Shinoda, Ai; Fukunaga, Yuichi; Kawahara, Akihiko; Karasuyama, Kazuyuki; Fukushi, Jun-Ichi; Iwamoto, Yukihide; Kuwano, Michihiko; Ono, Mayumi.

In: Scientific reports, Vol. 6, 19470, 18.01.2016.

Research output: Contribution to journalArticle

Watari, K, Shibata, T, Nabeshima, H, Shinoda, A, Fukunaga, Y, Kawahara, A, Karasuyama, K, Fukushi, J-I, Iwamoto, Y, Kuwano, M & Ono, M 2016, 'Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice', Scientific reports, vol. 6, 19470. https://doi.org/10.1038/srep19470
Watari, Kosuke ; Shibata, Tomohiro ; Nabeshima, Hiroshi ; Shinoda, Ai ; Fukunaga, Yuichi ; Kawahara, Akihiko ; Karasuyama, Kazuyuki ; Fukushi, Jun-Ichi ; Iwamoto, Yukihide ; Kuwano, Michihiko ; Ono, Mayumi. / Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice. In: Scientific reports. 2016 ; Vol. 6.
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