Impaired erythrocyte filterability of spontaneously hypertensive rats: Investigation by Nickel Mesh filtration technique

Kyoko Ariyoshi, Toru Maruyama, Keita Odashiro, Koichi Akashi, Takehiko Fujino, Nobuhiro Uyesaka

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hypertension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible nickel mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P<0.005, and 71.1±3.9% in 18 weeks of age, P<0.005, respectively). This impairment in SHR could not be explained by the mean corpuscular volume or mean corpuscular hemoglobin concentration of erythrocytes, but the erythrocyte count was significantly (P<0.005) greater in SHR than in the age-matched WKY. Conclusions: Although the precise mechanisms remain to be elucidated, markedly impaired erythrocyte filter-ability in SHR is considered to contribute to the development and maintenance of genetic hypertension.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalCirculation Journal
Volume74
Issue number1
DOIs
Publication statusPublished - Jan 1 2010

Fingerprint

Erythrocyte Deformability
Inbred SHR Rats
Nickel
Inbred WKY Rats
Erythrocyte Indices
Hypertension
Erythrocytes
Erythrocyte Count
Microcirculation
Tail
Maintenance
Blood Pressure

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Impaired erythrocyte filterability of spontaneously hypertensive rats : Investigation by Nickel Mesh filtration technique. / Ariyoshi, Kyoko; Maruyama, Toru; Odashiro, Keita; Akashi, Koichi; Fujino, Takehiko; Uyesaka, Nobuhiro.

In: Circulation Journal, Vol. 74, No. 1, 01.01.2010, p. 129-136.

Research output: Contribution to journalArticle

Ariyoshi, Kyoko ; Maruyama, Toru ; Odashiro, Keita ; Akashi, Koichi ; Fujino, Takehiko ; Uyesaka, Nobuhiro. / Impaired erythrocyte filterability of spontaneously hypertensive rats : Investigation by Nickel Mesh filtration technique. In: Circulation Journal. 2010 ; Vol. 74, No. 1. pp. 129-136.
@article{4c39e990c6464f6b9c14d5c8f8e6fe7f,
title = "Impaired erythrocyte filterability of spontaneously hypertensive rats: Investigation by Nickel Mesh filtration technique",
abstract = "Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hypertension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible nickel mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5{\%}) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3{\%}) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2{\%} in 7 weeks of age, P<0.005, and 71.1±3.9{\%} in 18 weeks of age, P<0.005, respectively). This impairment in SHR could not be explained by the mean corpuscular volume or mean corpuscular hemoglobin concentration of erythrocytes, but the erythrocyte count was significantly (P<0.005) greater in SHR than in the age-matched WKY. Conclusions: Although the precise mechanisms remain to be elucidated, markedly impaired erythrocyte filter-ability in SHR is considered to contribute to the development and maintenance of genetic hypertension.",
author = "Kyoko Ariyoshi and Toru Maruyama and Keita Odashiro and Koichi Akashi and Takehiko Fujino and Nobuhiro Uyesaka",
year = "2010",
month = "1",
day = "1",
doi = "10.1253/circj.CJ-09-0252",
language = "English",
volume = "74",
pages = "129--136",
journal = "Circulation Journal",
issn = "1346-9843",
publisher = "Japanese Circulation Society",
number = "1",

}

TY - JOUR

T1 - Impaired erythrocyte filterability of spontaneously hypertensive rats

T2 - Investigation by Nickel Mesh filtration technique

AU - Ariyoshi, Kyoko

AU - Maruyama, Toru

AU - Odashiro, Keita

AU - Akashi, Koichi

AU - Fujino, Takehiko

AU - Uyesaka, Nobuhiro

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hypertension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible nickel mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P<0.005, and 71.1±3.9% in 18 weeks of age, P<0.005, respectively). This impairment in SHR could not be explained by the mean corpuscular volume or mean corpuscular hemoglobin concentration of erythrocytes, but the erythrocyte count was significantly (P<0.005) greater in SHR than in the age-matched WKY. Conclusions: Although the precise mechanisms remain to be elucidated, markedly impaired erythrocyte filter-ability in SHR is considered to contribute to the development and maintenance of genetic hypertension.

AB - Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hypertension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible nickel mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P<0.005, and 71.1±3.9% in 18 weeks of age, P<0.005, respectively). This impairment in SHR could not be explained by the mean corpuscular volume or mean corpuscular hemoglobin concentration of erythrocytes, but the erythrocyte count was significantly (P<0.005) greater in SHR than in the age-matched WKY. Conclusions: Although the precise mechanisms remain to be elucidated, markedly impaired erythrocyte filter-ability in SHR is considered to contribute to the development and maintenance of genetic hypertension.

UR - http://www.scopus.com/inward/record.url?scp=75149148693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75149148693&partnerID=8YFLogxK

U2 - 10.1253/circj.CJ-09-0252

DO - 10.1253/circj.CJ-09-0252

M3 - Article

C2 - 19952438

AN - SCOPUS:75149148693

VL - 74

SP - 129

EP - 136

JO - Circulation Journal

JF - Circulation Journal

SN - 1346-9843

IS - 1

ER -