Impaired protection against Mycobacterium bovis bacillus Calmette-Guérin infection in IL-15-deficient mice

Kimika Saito, Toshiki Yajima, Shino Kumabe, Takehiko Doi, Hisakata Yamada, Subash Sad, Hao Shen, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    To investigate the potential role of endogenous IL-15 in mycobacterial infection, we examined protective immunity in IL-15-deficient (IL-15 -/-) mice after infection with Mycobacterium bovis bacillus Calmette-Guérin (BCG) or recombinant OVA-expressing BCG (rBCG-OVA). IL-15-/- mice exhibited an impaired protection in the lung on day 120 after BCG infection as assessed by bacterial growth. CD4+ Th1 response capable of producing IFN-γ was normally detected in spleen and lung of IL-15-/- mice on day 120 after infection. Although Ag-specific CD8 responses capable of producing IFN-γ and exhibiting cytotoxic activity were detected in the lung on day 21 after infection with rBCG-OVA, the responses were severely impaired on days 70 and 120 in IL-15 -/- mice. The degree of proliferation of Ag-specific CD8+ T cells in IL-15-/- mice was similar to that in wild-type mice during the course of infection with rBCG-OVA, whereas sensitivity to apoptosis of Ag-specific CD8+ T cells significantly increased in IL-15 -/- mice. These results suggest that IL-15 plays an important role in the development of long-lasting protective immunity to BCG infection via sustaining CD8 responses in the lung.

    Original languageEnglish
    Pages (from-to)2496-2504
    Number of pages9
    JournalJournal of Immunology
    Volume176
    Issue number4
    DOIs
    Publication statusPublished - Feb 15 2006

    Fingerprint

    Interleukin-15
    Mycobacterium bovis
    Bacillus
    Infection
    Lung
    Immunity
    T-Lymphocytes
    Spleen
    Apoptosis
    Growth

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Impaired protection against Mycobacterium bovis bacillus Calmette-Guérin infection in IL-15-deficient mice. / Saito, Kimika; Yajima, Toshiki; Kumabe, Shino; Doi, Takehiko; Yamada, Hisakata; Sad, Subash; Shen, Hao; Yoshikai, Yasunobu.

    In: Journal of Immunology, Vol. 176, No. 4, 15.02.2006, p. 2496-2504.

    Research output: Contribution to journalArticle

    Saito, K, Yajima, T, Kumabe, S, Doi, T, Yamada, H, Sad, S, Shen, H & Yoshikai, Y 2006, 'Impaired protection against Mycobacterium bovis bacillus Calmette-Guérin infection in IL-15-deficient mice', Journal of Immunology, vol. 176, no. 4, pp. 2496-2504. https://doi.org/10.4049/jimmunol.176.4.2496
    Saito, Kimika ; Yajima, Toshiki ; Kumabe, Shino ; Doi, Takehiko ; Yamada, Hisakata ; Sad, Subash ; Shen, Hao ; Yoshikai, Yasunobu. / Impaired protection against Mycobacterium bovis bacillus Calmette-Guérin infection in IL-15-deficient mice. In: Journal of Immunology. 2006 ; Vol. 176, No. 4. pp. 2496-2504.
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