Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice

Hiroshi Yamatani, Kaori Kohzuma, Michiharu Nakano, Tsuneaki Takami, Yusuke Kato, Yoriko Hayashi, Yuki Monden, Yutaka Okumoto, Tomoko Abe, Toshihiro Kumamaru, Ayumi Tanaka, Wataru Sakamoto, Makoto Kusaba

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Abstract

Chlorophyll is an essential molecule for acquiring light energy during photosynthesis. Mutations that result in chlorophyll retention during leaf senescence are called - € stay-green' mutants. One of the several types of stay-green mutants, Type E, accumulates high levels of chlorophyll in the pre-senescent leaves, resulting in delayed yellowing. We isolated delayed yellowing1-1 (dye1-1), a rice mutant whose yellowing is delayed in the field. dye1-1 accumulated more chlorophyll than the wild-type in the pre-senescent and senescent leaves, but did not retain leaf functionality in the - €- senescent green leaves', suggesting that dye1-1 is a Type E stay-green mutant. Positional cloning revealed that DYE1 encodes Lhca4, a subunit of the light-harvesting complex I (LHCI). In dye1-1, amino acid substitution occurs at the location of a highly conserved amino acid residue involved in pigment binding; indeed, a severely impaired structure of the PSI-LHCI super-complex in dye1-1 was observed in a blue native PAGE analysis. Nevertheless, the biomass and carbon assimilation rate of dye1-1 were comparable to those in the wild-type. Interestingly, Lhcb1, a trimeric LHCII protein, was highly accumulated in dye1-1, in the chlorophyll-protein complexes. The high accumulation of LHCII in the LHCI mutant dye1 suggests a novel functional interaction between LHCI and LHCII.

Original languageEnglish
Pages (from-to)1027-1035
Number of pages9
JournalJournal of Experimental Botany
Volume69
Issue number5
DOIs
Publication statusPublished - Feb 23 2018

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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    Yamatani, H., Kohzuma, K., Nakano, M., Takami, T., Kato, Y., Hayashi, Y., Monden, Y., Okumoto, Y., Abe, T., Kumamaru, T., Tanaka, A., Sakamoto, W., & Kusaba, M. (2018). Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice. Journal of Experimental Botany, 69(5), 1027-1035. https://doi.org/10.1093/jxb/erx468